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However, the roles of <jats:italic>ZEB1<\/jats:italic> and type\u2010H vessels in steroid\u2010induced osteonecrosis of the femoral head (SONFH) are unclear.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Human femoral heads were collected to detect the expression of <jats:italic>ZEB1<\/jats:italic> and the levels of type\u2010H vessels. Then, the SONFH model was developed by injecting C57BL\/6 mice with lipopolysaccharide and methylprednisolone. Micro\u2010computed tomography, angiography, double calcein labeling, immunofluorescence, immunohistochemistry, quantitative real\u2010time polymerase chain reaction, and Western blotting were performed to detect the expression of <jats:italic>ZEB1<\/jats:italic>, the <jats:italic>Wnt\/\u03b2\u2010catenin<\/jats:italic> pathway, type\u2010H vessels, and the extent to which <jats:italic>ZEB1<\/jats:italic> mediates angiogenesis and osteogenesis. Human umbilical vein endothelial cells were also used to explore the relationship between <jats:italic>ZEB1<\/jats:italic> and the <jats:italic>Wnt\/\u03b2\u2010catenin<\/jats:italic> pathway.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We found that <jats:italic>ZEB1<\/jats:italic> expression and the formation of type\u2010H vessels decreased in SONFH patients and in a mouse model. The number of vascular endothelial growth factors in the femoral heads also decreased. Moreover, the bone mineral density, trabecular number, mineral apposition rate, and expression of genes related to osteogenesis decreased. After <jats:italic>ZEB1<\/jats:italic> knockdown, angiogenesis and osteogenesis decreased. However, the numbers of type\u2010H vessels and the extent of angiogenesis and osteogenesis improved after activation of the <jats:italic>Wnt\/\u03b2\u2010catenin<\/jats:italic> pathway.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The\u00a0ZEB1 expression decreased in SONFH, causing a decrease in type\u2010H vessel, and it mediated angiogenesis and osteogenesis by regulating the <jats:italic>Wnt\/\u03b2\u2010catenin<\/jats:italic> pathway, ultimately accelerating the process of SONFH.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ame2.12507","type":"journal-article","created":{"date-parts":[[2024,12,17]],"date-time":"2024-12-17T06:23:13Z","timestamp":1734416593000},"page":"802-815","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["The decrease in zinc\u2010finger E\u2010box\u2010binding homeobox\u20101 could accelerate steroid\u2010induced osteonecrosis of the femoral head by repressing 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