{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T20:02:48Z","timestamp":1792008168011,"version":"4.4.0"},"reference-count":33,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,1,23]],"date-time":"2025-01-23T00:00:00Z","timestamp":1737590400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Anim Models and Exp Med"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Indole phytoalexins, plant\u2010derived compounds present in cruciferous vegetables, have demonstrated anticancer properties. Brassinin (BSN), derived from\n                      <jats:italic>Brassica campestris<\/jats:italic>\n                      L. var. campestris, is known for its potent antitumor effects on various cancers. However, the role of ferroptosis in regulating the antitumor effects of BSN has not been fully elucidated.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      The components of\n                      <jats:italic>B. campestris<\/jats:italic>\n                      L. against colorectal cancer (CRC) were analyzed by network pharmacology. CCK\u20108 assay and colony formation assay detected cell viability induced by BSN. Molecular docking verified the binding of BSN to the target protein. Western blot and reverse transcription\u2013quantitative polymerase chain reaction (RT\u2010qPCR) assay revealed whether BSN can inactivate the NRF2 signaling and inhibit the expression of p62 and HO\u20101. The RKO\u2010xenograft tumor models were established and then were treated by 75 or 150\u2009mg\/kg BSN to verify the antitumor efficacy and side effects of BSN.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Network pharmacology suggested that BSN is the most important component of\n                      <jats:italic>B. campestris<\/jats:italic>\n                      L. against CRC. BSN inhibits CRC cell viability in a dose\u2010 and time\u2010dependent manner. Furthermore, this inhibitory effect is associated with the induction of ferroptosis, as BSN suppresses the cell viability of CRC by inducing GPX4\u2010regulated ferroptosis. BSN may bind to NRF2 protein to inactivate the NRF2 signaling, inhibiting the expression of p62 and HO\u20101. Importantly, a low dose or a high dose of BSN significantly reduced the tumor growth in\u00a0vivo.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our findings reveal that BSN blocks CRC growth by inducing p62\/NRF2\/GPX4\u2010regulated ferroptosis, which may be a novel lead compound for tumor treatment.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ame2.12521","type":"journal-article","created":{"date-parts":[[2025,1,23]],"date-time":"2025-01-23T07:10:13Z","timestamp":1737616213000},"page":"1155-1165","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Brassinin from\n                    <i>Brassica campestris<\/i>\n                    L. inhibits colorectal cancer by inducing p62\/\n                    <scp>NRF2<\/scp>\n                    \/\n                    <scp>GPX4<\/scp>\n                    \u2010regulated ferroptosis"],"prefix":"10.1002","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9589-5676","authenticated-orcid":false,"given":"Shi\u2010Yuan","family":"Wen","sequence":"first","affiliation":[{"name":"College of Basic Medical Sciences, Shanxi Medical University  Taiyuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui\u2010Rui","family":"Gao","sequence":"additional","affiliation":[{"name":"College of Traditional Chinese Medicine and Food Engineering, Shanxi University of Chinese Medicine  Taiyuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan\u2010Yan","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Medicine Jiangsu University  Zhenjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi\u2010Jie","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Traditional Chinese Medicine and Food 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