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By performing serial allogeneic hepatocyte transplantation in CRISPR\/Cas9\u2010mediated <jats:italic>Fah<\/jats:italic>\u2010knockout pigs, we successfully achieved large\u2010scale expansion of hepatocytes while maintaining their authentic biological characteristics. Particularly, the established model enables sustained in\u00a0vivo liver reconstruction, concurrently ameliorating hepatic fibrosis and demonstrating functional microenvironmental remodeling. Moreover, through comprehensive single\u2010cell transcriptomic profiling of 52\u2009418 hepatocytes across transplant generations (F0\u2013F2), we discovered that the cellular composition of these transplanted hepatocytes is similar to that of wild\u2010type hepatocytes. The regenerated liver exhibits all six major hepatic cell types identical to the wild\u2010type counterparts, with the characteristic lobular zonation patterns well preserved. Our research provides valuable insights into the large\u2010scale expansion of physiologically functional hepatocytes in\u00a0vivo without compromising their biological properties. 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