{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T03:21:49Z","timestamp":1779333709893,"version":"3.51.4"},"reference-count":41,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,6,30]],"date-time":"2025-06-30T00:00:00Z","timestamp":1751241600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Anim Models and Exp Med"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Silicosis is an occupational lung disease that is caused by chronic exposure to silica dust. Silica\u2010exposed workers are at higher risk of developing TB, resulting in lung fibrosis and significant respiratory dysfunction. Diosgenin is a steroidal saponin that has been shown to exert a therapeutic effect on lung injury. Therefore, we investigated the potential efficacy of diosgenin in treating silicotuberculosis by evaluating its effectiveness against <jats:italic>Mycobacterium smegmatis<\/jats:italic>, as well as its antifibrotic and antioxidant effects in silica\u2010induced TB in rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Silicosis was induced by intratracheal instillation of 50\u2009mg\/kg crystalline silica in Sprague\u2013Dawley rats. Rats were grouped into 7 (10 per group). Different doses of diosgenin (1, 10, and 20\u2009mg\/kg) and saline were administered for 30\u2009days. Afterwards, five rats from each group were sacrificed, and the five remaining rats in each group, except the control, received <jats:italic>Mycobacterium smegmatis<\/jats:italic>. Treatment continued until the 50th day, and the animals were sacrificed at the end of the experiment. The result was analyzed using a one\u2010way analysis of variance (ANOVA) with GraphPad Prism.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>At a half\u2010maximal inhibition concentration of 0.006043\u2009\u03bcg\/mL, diosgenin inhibited the growth of <jats:italic>Mycobacterium smegmatis<\/jats:italic>. Oxidative stress markers such as malondialdehyde were significantly reduced. The health\u2010enhancing effects of catalase and superoxide dismutase were elevated. Additionally, histological findings demonstrated a significant improvement in respiratory function following diosgenin treatment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Diosgenin treatment inhibited the growth of <jats:italic>Mycobacterium smegmatis,<\/jats:italic> leading to a reduction in the susceptibility of rats to infection and improved pulmonary function through its antioxidant effect.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ame2.70055","type":"journal-article","created":{"date-parts":[[2025,6,30]],"date-time":"2025-06-30T05:09:33Z","timestamp":1751260173000},"page":"1186-1195","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Diosgenin ameliorates silica\u2010induced tuberculosis in rats"],"prefix":"10.1002","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-6246-7035","authenticated-orcid":false,"given":"Williams 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