{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T03:17:12Z","timestamp":1792552632182,"version":"4.5.0"},"reference-count":29,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T00:00:00Z","timestamp":1764633600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T00:00:00Z","timestamp":1764633600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Anim Models and Exp Med"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Osteoclasts are essential for maintaining healthy bone. Pathological elevation of osteoclastogenesis or osteoclast activity can cause osteoporosis and increase the risk of bone fracture. However, a few options are available for directly measuring osteoclast activity in\u00a0vivo to test interventions that may affect osteoclasts. Here, we describe an in\u00a0vivo method to measure osteoclast\u2010mediated bone loss targeted at normal mouse calvaria. The method employs a novel procedure for measuring osteoclast resorption pits using micro\u2010computed tomography. The potential utility of this mouse calvaria model to assess therapies targeting osteoclasts was validated using zoledronic acid, which is a nitrogen\u2010containing bisphosphonate drug used to treat osteoporosis.<\/jats:p>","DOI":"10.1002\/ame2.70112","type":"journal-article","created":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T10:12:12Z","timestamp":1764670332000},"page":"41-49","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["An in\u00a0vivo assay for osteoclast activity using mouse calvaria"],"prefix":"10.1002","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5022-1758","authenticated-orcid":false,"given":"Christopher","family":"Grieg","sequence":"first","affiliation":[{"name":"Department of Orthopaedics Rutgers\u2010New Jersey Medical School  Newark New Jersey USA"},{"name":"Rutgers\u2010School of Graduate Studies, Newark Health Sciences Campus  Newark New Jersey USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maya Deza","family":"Culbertson","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics Rutgers\u2010New Jersey Medical School  Newark New Jersey USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0282-1104","authenticated-orcid":false,"given":"J. Patrick","family":"O'Connor","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics Rutgers\u2010New Jersey Medical School  Newark New Jersey USA"},{"name":"Rutgers\u2010School of Graduate Studies, Newark Health Sciences Campus  Newark New Jersey USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,12,2]]},"reference":[{"key":"e_1_2_14_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells9092073"},{"key":"e_1_2_14_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecl.2021.07.005"},{"key":"e_1_2_14_4_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/421746"},{"key":"e_1_2_14_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg1122"},{"key":"e_1_2_14_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bdj.4805313"},{"key":"e_1_2_14_7_1","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2011.362"},{"key":"e_1_2_14_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00223-013-9786-7"},{"key":"e_1_2_14_9_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2000.71.4.586"},{"key":"e_1_2_14_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.1543"},{"issue":"2","key":"e_1_2_14_11_1","first-page":"311","article-title":"Noninvasive imaging of osteoclasts in parathyroid hormone\u2010induced osteolysis using a 64Cu\u2010labeled RGD peptide","volume":"48","author":"Sprague JE","year":"2007","journal-title":"J Nucl Med"},{"key":"e_1_2_14_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.5650100503"},{"key":"e_1_2_14_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.2974"},{"key":"e_1_2_14_14_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI13890"},{"key":"e_1_2_14_15_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI8905"},{"key":"e_1_2_14_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-024-08163-9"},{"key":"e_1_2_14_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/brv.12975"},{"key":"e_1_2_14_18_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1900665"},{"key":"e_1_2_14_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200636534"},{"key":"e_1_2_14_20_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.18.7260"},{"key":"e_1_2_14_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s12276-020-0406-0"},{"key":"e_1_2_14_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2009.03.005"},{"key":"e_1_2_14_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells9092086"},{"key":"e_1_2_14_24_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0086757"},{"key":"e_1_2_14_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00229061"},{"key":"e_1_2_14_26_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.186.4.489"},{"key":"e_1_2_14_27_1","doi-asserted-by":"publisher","DOI":"10.1210\/me.2009-0354"},{"key":"e_1_2_14_28_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2018-00220"},{"key":"e_1_2_14_29_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI119172"},{"key":"e_1_2_14_30_1","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.b18-00026"}],"container-title":["Animal Models and Experimental Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ame2.70112","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/ame2.70112","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ame2.70112","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T10:51:31Z","timestamp":1771239091000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ame2.70112"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,2]]},"references-count":29,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["10.1002\/ame2.70112"],"URL":"https:\/\/doi.org\/10.1002\/ame2.70112","archive":["Portico"],"relation":{},"ISSN":["2576-2095","2576-2095"],"issn-type":[{"value":"2576-2095","type":"print"},{"value":"2576-2095","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12,2]]},"assertion":[{"value":"2025-08-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-29","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-02","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}