{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T08:50:46Z","timestamp":1773391846345,"version":"3.50.1"},"reference-count":23,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T00:00:00Z","timestamp":1768348800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T00:00:00Z","timestamp":1768348800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Anim Models and Exp Med"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The traditional method of heterotopic abdominal heart transplantation (HTx) involves cross\u2010clamping the inferior vena cava, which inevitably leads to bilateral lower limb ischemia (LI). This study first aimed to investigate the impact of LI on renal function in rats subjected to unilateral nephrectomy (UNx). Second, a modified method utilizing renal vessel\u2013assisted anastomosis in rats with left UNx was compared with the traditional method for abdominal HTx.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Male Sprague\u2013Dawley rats were utilized as subjects for both experimental phases. In experiment 1, the animals were divided into four groups: sham operation group; LI group\u2014rats undergoing occlusion of the abdominal aorta and vena cava below the renal vessels; UNx group\u2014rats with left UNx; and LI\u2009+\u2009UNx group. All operated animals were monitored for up to 7\u2009days for biochemical markers, renal histopathology, and survival rates. In experiment 2, we introduced the renal vessel\u2013assisted method as the experimental group and compared it against the traditional method as the control within rat heterotopic HTx models. We assessed operative characteristics, echocardiography results, histological findings, and graft survival.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>First, LI resulted in acute kidney dysfunction characterized by a decrease in 7\u2010day survival rates and creatinine clearance rates in both the LI and LI\u2009+\u2009UNx groups compared to the sham operation and UNx groups. Particularly, histopathological damage in the kidney and liver did not exhibit significant effects during this period. Second, the implementation of the renal vessel\u2013assisted method significantly reduced bleeding volume at suture sites and enhanced the 7\u2010day survival rate compared to the traditional method.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Acute kidney injury was induced by LI postoperation in treated rats. The renal vessel\u2013assisted method demonstrated its effectiveness as a superior alternative that mitigates complications associated with the traditional method.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ame2.70113","type":"journal-article","created":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T08:23:03Z","timestamp":1768378983000},"page":"407-415","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Renal vessel\u2013assisted anastomosis improves the abdominal heart transplant model in rats without bilateral lower limb ischemia"],"prefix":"10.1002","volume":"9","author":[{"given":"Meng","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Cardiovascular Surgery Chest Hospital, Tianjin University  Tianjin People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wuxia","family":"Wang","sequence":"additional","affiliation":[{"name":"Health Training Center, Medical Service Training Center The 983rd Hospital of the Joint Logistics Support Force of the Chinese People's Liberation Army  Tianjin People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-5770-0014","authenticated-orcid":false,"given":"Xunfeng","family":"Zou","sequence":"additional","affiliation":[{"name":"Thyroid Breast and Hernia Surgery Tianjin First Central Hospital  Tianjin People's Republic of China"},{"name":"Organ Transplant Department, Institute of Transplantation Nankai University  Tianjin People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,1,14]]},"reference":[{"issue":"4","key":"e_1_2_10_2_1","first-page":"165","article-title":"Heart homotransplantation in the rat","volume":"30","author":"Tomita F","year":"1966","journal-title":"Sapporo Igaku 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