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While conventional rat EVLP systems utilize closed\u2010loop dual cannulation of pulmonary artery (PA) and vein, the effect of the simplified model using single PA cannulation with passive venous drainage is unknown.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We developed two EVLP models in rats: a semi\u2010closed circuit with PA\u2010only cannulation and left atrial incision for passive venous drainage (SC\u2010EVLP), and a closed circuit employing both arterial and venous cannulation (C\u2010EVLP). Donor lungs were perfused for a defined duration and subsequently orthotopically transplanted. We evaluated pulmonary function parameters, histopathological injury scores, inflammatory cytokine levels, and apoptotic marker expression at the end of perfusion and posttransplantation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Compared to the conventional EVLP, the SC\u2010EVLP group exhibited significantly lower PA pressure and improved dynamic lung compliance throughout perfusion. Although the levels of tumor necrosis factor\u2010\u03b1 in the perfusate were higher in the SC\u2010EVLP group, other cytokine levels in the perfusate and bronchoalveolar lavage fluid exhibited no significant differences. Pulmonary edema was reduced in the SC\u2010EVLP group, as indicated by a lower lung wet\u2010to\u2010dry ratio. After transplantation, lungs from the SC\u2010EVLP group exhibited lower histological injury scores, reduced apoptosis, and decreased serum cytokine levels, suggesting attenuated inflammation and tissue damage.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>In a rat model, single PA cannulation with passive venous drainage reduced pulmonary edema during EVLP and reduced lung injury and systemic inflammation after transplantation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ame2.70122","type":"journal-article","created":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T07:31:13Z","timestamp":1768807873000},"page":"183-192","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Comparative evaluation of single\u2010artery cannulation with passive venous drainage versus traditional dual\u2010cannula ex\u00a0vivo lung perfusion in a rat model"],"prefix":"10.1002","volume":"9","author":[{"given":"Ming","family":"Ni","sequence":"first","affiliation":[{"name":"Department of Thoracic Surgery The First Affiliated Hospital of Xi'an Jiaotong University  Xi'an China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fei","family":"Xue","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery The First Affiliated Hospital of Xi'an Jiaotong University  Xi'an China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuanpeng","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery The First Affiliated Hospital of Xi'an Jiaotong University  Xi'an China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chenxi","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery The First Affiliated Hospital of Xi'an Jiaotong University  Xi'an 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