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Mice in the TMAO and TMAO+MICT groups received daily gavage of high\u2010dose TMAO for 8\u2009weeks, whereas those in the Nor+MICT and TMAO+MICT groups underwent MICT for 8\u2009weeks (60\u2009min per session, 5\u2009days per week, at 50% maximal running capacity). Cardiac function was evaluated using ultrasound, myocardial histology was examined using hematoxylin and eosin (HE) staining, and nuclear magnetic resonance (NMR)\u2010based metabolomics was employed for multivariate statistical and metabolic pathway analyses.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Relative to the Nor group, TMAO\u2010treated mice exhibited significant weight loss, elevated heart rate, and reduced ejection fraction and left ventricular fractional shortening, indicating cardiac impairment. Importantly, the TMAO+MICT group demonstrated significant improvements in these parameters compared to the TMAO group, alongside distinct alterations in myocardial metabolic profiles. TMAO altered five metabolic pathways relative to controls, whereas MICT induced significant changes in three pathways in TMAO\u2010treated mice.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Eight weeks of high\u2010dose TMAO administration induced significant cardiac dysfunction in mice, which was effectively mitigated by MICT intervention. Consequently, this animal model serves as a valuable tool for investigating the mechanisms underlying the impact of MICT on cardiovascular diseases.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ame2.70125","type":"journal-article","created":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T04:38:43Z","timestamp":1769056723000},"page":"142-153","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Establishment of a mouse model of\n                    <scp>TMAO<\/scp>\n                    \u2010induced cardiac injury and application of\n                    <scp>MICT<\/scp>\n                    intervention"],"prefix":"10.1002","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-8719-9907","authenticated-orcid":false,"given":"Zhongping","family":"Xie","sequence":"first","affiliation":[{"name":"Physical Education Department Xiamen University of Technology  Xiamen 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