{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T08:51:17Z","timestamp":1773391877947,"version":"3.50.1"},"reference-count":61,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T00:00:00Z","timestamp":1772150400000},"content-version":"vor","delay-in-days":26,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100021954","name":"Tour de Cure","doi-asserted-by":"publisher","award":["RSP\u2010253\u201018\/19"],"award-info":[{"award-number":["RSP\u2010253\u201018\/19"]}],"id":[{"id":"10.13039\/501100021954","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Anim Models and Exp Med"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Realistic models for cancer research representing disease progression that commensurately respond to therapeutics consistent with clinical observation are the holy grail for pre\u2010clinical research and screening. Although such an ideal is elusive, well\u2010characterized in\u00a0vivo models facilitate our understanding of disease, progression, and therapeutic opportunities. Here, we characterize a commonly used syngeneic BALB\/c mouse model of triple negative breast cancer (4T1) after establishing tumors in their flanks. Tumors developed at the subcutaneous injection site for all experimental mice and their volumes were monitored. We quantified a rare subset of breast cancer stem\u2010like cells (CSCs), classified as CD44\n                    <jats:sup>+<\/jats:sup>\n                    \/CD24\n                    <jats:sup>\u2212<\/jats:sup>\n                    phenotypes in in\u00a0vitro and ex\u00a0vivo cell populations. Chromosome numbers in ex\u00a0vivo metaphase cells were greater than cells cultured in\u00a0vitro (89.4\u2009\u00b1\u20093.4, range of 70\u2013132 and 82.6\u2009\u00b1\u20091.1, range of 70\u2013128; respectively). Further, we observed different types of chromosome aberrations, including gap, deletion, exchange, interstitial deletion, terminal deletion, ring, dicentric, and Robertsonian translocations. For both sources of cells, the number of aberrations was dominated by deletions, terminal deletions, and Robertsonian translocations. Ex\u00a0vivo cells exhibited greater prevalence of deletions and terminal deletions, whereas in\u00a0vitro cells displayed more ring aberrations and Robertsonian translocations. In conclusion, we successfully characterized cancer cells from a syngeneic mouse model of breast cancer in terms of rare CSC proportion and a variety of chromosomal aberrations, which is useful for understanding tumor traits associated with cancer development and therapeutic action. The data act as a valuable resource for other studies using the 4T1 BALB\/c model.\n                  <\/jats:p>","DOI":"10.1002\/ame2.70156","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T17:41:10Z","timestamp":1772991670000},"page":"367-377","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Characterizing a murine breast cancer mouse model reveals chromosomal abnormalities in structure and number of single\u2010cell clones and the presence of rare cancer stem cell\u2010like phenotypes"],"prefix":"10.1002","volume":"9","author":[{"given":"Quy Van\u2010Chanh","family":"Le","sequence":"first","affiliation":[{"name":"Future Industries Institute Adelaide University  Mawson Lakes South Australia Australia"},{"name":"School of Pharmacy and Biomedical Science, College of Health Adelaide University  Adelaide South Australia Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zsuzsa S.","family":"Kocsis","sequence":"additional","affiliation":[{"name":"Department of Radiobiology and Diagnostic Onco\u2010Cytogenetics, Centre of Radiotherapy National Institute of Oncology  Budapest Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sisi","family":"Liang","sequence":"additional","affiliation":[{"name":"Future Industries Institute Adelaide University  Mawson Lakes South Australia Australia"},{"name":"School of Pharmacy and Biomedical Science, College of Health Adelaide University  Adelaide South Australia Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanrui","family":"Li","sequence":"additional","affiliation":[{"name":"Future Industries Institute Adelaide University  Mawson Lakes South Australia Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Briony L.","family":"Gliddon","sequence":"additional","affiliation":[{"name":"Centre for Cancer Biology Adelaide University and SA Pathology  Adelaide South Australia Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sheree","family":"Bailey","sequence":"additional","affiliation":[{"name":"School of Pharmacy and Biomedical Science, College of Health Adelaide University  Adelaide South Australia Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anh Thai\u2010Quynh","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Future Industries Institute Adelaide University  Mawson Lakes South Australia Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tyron","family":"Turnbull","sequence":"additional","affiliation":[{"name":"School of Physics, Chemistry &amp; Earth Science, College of Science Adelaide University  Adelaide South Australia Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stuart M.","family":"Pitson","sequence":"additional","affiliation":[{"name":"Centre for Cancer Biology Adelaide University and SA Pathology  Adelaide South Australia Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zsolt","family":"Jur\u00e1nyi","sequence":"additional","affiliation":[{"name":"Department of Radiobiology and Diagnostic Onco\u2010Cytogenetics, Centre of Radiotherapy National Institute of Oncology  Budapest Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hien","family":"Le","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology Royal Adelaide Hospital  Adelaide South Australia Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3886-9516","authenticated-orcid":false,"given":"Ivan","family":"Kempson","sequence":"additional","affiliation":[{"name":"Future Industries Institute Adelaide University  Mawson Lakes South Australia Australia"},{"name":"Centre for Pharmaceutical Innovation, School of Pharmacy and Biomedical Science, College of Health Adelaide University  Adelaide South Australia Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,2,27]]},"reference":[{"issue":"1","key":"e_1_2_11_2_1","article-title":"Mouse 4T1 breast tumor model","volume":"39","author":"Pulaski BA","year":"2001","journal-title":"Curr Protoc 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H","year":"2009","journal-title":"Anticancer Res"},{"key":"e_1_2_11_18_1","article-title":"Sample preparation for mass cytometry analysis","volume":"122","author":"McCarthy RL","year":"2017","journal-title":"J Vis 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