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We established an omohyoid muscle\u2010based PNS rat model combined with MV. Bilateral nerves were exposed within 20\u2009\u00b1\u20092\u2009min by transection at the intermediate tendon of omohyoid muscle, minimizing trauma and bleeding. Threshold stimulation (0.6\u2009\u00b1\u20090.2\u2009mA) correlated with body weight. Ventilator\u2010synchronized stimulation increased compound muscle action potentials by ~30%, whereas histology confirmed intact nerve. Physiological parameters remained stable throughout ventilation. This model provides a safe and scalable platform for mechanistic and preclinical studies on PNS\u2010mediated protection against MV\u2010induced organ injury.<\/jats:p>","DOI":"10.1002\/ame2.70168","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T16:59:00Z","timestamp":1772989140000},"page":"416-421","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A preclinical rat model for bilateral phrenic nerve stimulation during mechanical ventilation"],"prefix":"10.1002","volume":"9","author":[{"given":"Jingyi","family":"Li","sequence":"first","affiliation":[{"name":"Department of Neurosurgery, Intensive Care Unit Sanbo Brain Hospital, Capital Medical University  Beijing China"},{"name":"Laboratory for Clinical Medicine Capital Medical University  Beijing 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