{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T22:20:37Z","timestamp":1783117237126,"version":"3.54.6"},"reference-count":47,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T00:00:00Z","timestamp":1774569600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T00:00:00Z","timestamp":1774569600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004300","name":"Senior Talent Foundation of Jiangsu University","doi-asserted-by":"publisher","award":["5501290013"],"award-info":[{"award-number":["5501290013"]}],"id":[{"id":"10.13039\/501100004300","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Anim Models and Exp Med"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Human cytomegalovirus (HCMV) infection is related to the acceleration of transplant vascular sclerosis, atherosclerosis, and coronary restenosis. A shared theme of these vascular illnesses is pathologic angiogenesis. Nevertheless, how HCMV infection causes angiogenesis is not fully understood. Human serum contains HCMV\u2010encoded miRNAs, and it is unclear whether these virus\u2010derived miRNAs can regulate angiogenesis. This research looks into HCMV\u2010encoded miRNA's role in angiogenesis of endothelial cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Endothelial cell proliferation was examined by CCK8 assay, and cell migration capability was established using a Transwell Boyden Chamber. Western blotting alongside luciferase reporter assay verified the direct regulation of FOXO3 by HCMV\u2010encoded miRNAs, including hcmv\u2010miR\u2010UL36\u20103p. hcmv\u2010miR\u2010UL36\u20103p's pro\u2010angiogenic action was examined by angiogenesis assays (in vivo) and capillary tube formation (in vitro), which were performed by giving C57BL\/6J mice subcutaneous Matrigel injections containing bFGF along with simultaneous injections of either hcmv\u2010miR\u2010UL36\u20103p or ncRNA once every 4\u2009days. After 8\u2009days, Matrigel plugs were examined.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>hcmv\u2010miR\u2010UL36\u20103p was upregulated in patients with atherosclerosis. Overexpression of hcmv\u2010miR\u2010UL36\u20103p enhanced capillary tube development, motility, and proliferation in endothelial cells. hcmv\u2010miR\u2010UL36\u20103p promoted endothelial cell tube formation through directly binding to and downregulating FOXO3. Experiments in mice further confirmed that hcmv\u2010miR\u2010UL36\u20103p promoted angiogenesis in\u00a0vivo.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The HCMV\u2010encoded miR\u2010UL36\u20103p can trigger angiogenesis in endothelial cells by targeting FOXO3. Our work provides a conceivable mechanism of how HCMV\u2010encoded miRNAs contribute to vascular illness.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ame2.70196","type":"journal-article","created":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T07:22:13Z","timestamp":1774596133000},"page":"980-990","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The HCMV\u2010encoded miR\u2010UL36\u20103p promotes angiogenesis of endothelial cells by downregulating FOXO3"],"prefix":"10.1002","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6461-1271","authenticated-orcid":false,"given":"Chen","family":"Wang","sequence":"first","affiliation":[{"name":"School of Pharmacy Jiangsu University  Zhenjiang Jiangsu China"},{"name":"State Key Laboratory of Pharmaceutical Biotechnology, Chinese Academy of Medical Sciences Research Unit of Extracellular RNA, Nanjing Drum Tower Hospital Center of Molecular Diagnostic and Therapy, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, NJU Advanced Institute of Life Sciences (NAILS), Institute of Artificial Intelligence Biomedicine, School of Life Sciences Nanjing University  Nanjing Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengyu","family":"Li","sequence":"additional","affiliation":[{"name":"School of Pharmacy Jiangsu University  Zhenjiang Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guosheng","family":"Li","sequence":"additional","affiliation":[{"name":"School of Pharmacy Jiangsu University  Zhenjiang Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Critical Care Medicine Affiliated Jinling Hospital, Medical School of Nanjing University  Nanjing Jiangsu 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