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However, routine detection methods such as traditional bacterial culture remain labor\u2010intensive and time\u2010consuming. The loop\u2010mediated isothermal amplification\u2010lateral flow dipstick (LAMP\u2013LFD) assay provides a self\u2010contained format for nucleic acid testing that combines speed, sensitivity, and specificity while minimizing aerosol contamination, making it well\u2010suited for point\u2010of\u2010need applications.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>This study aimed to develop a streamlined, on\u2010site detection platform capable of rapidly identifying both pathogens with high analytical accuracy, operational simplicity, and suitability for routine laboratory surveillance.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A LAMP\u2013LFD system was established for the detection of the two pathogens using primers designed against the conserved 16S\u201323S rRNA ITS region. The assay's specificity, sensitivity, repeatability, and limit of detection (LoD) were evaluated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The assay achieved detection limits of 1\u2009\u00d7\u200910\n                      <jats:sup>\u22124<\/jats:sup>\n                      \u2009ng\/\u03bcL for\n                      <jats:italic>R. pneumotropicus<\/jats:italic>\n                      and 1\u2009\u00d7\u200910\n                      <jats:sup>\u22125<\/jats:sup>\n                      \u2009ng\/\u03bcL for\n                      <jats:italic>R. heylii<\/jats:italic>\n                      , demonstrating significantly higher sensitivity than PCR. No cross\u2010reactivity was observed with 15 non\u2010target bacteria. Among 28 infected samples, the method detected 19 positives, compared with 21 by multiplex PCR and 23 by qPCR. In 842 laboratory animal samples, culture\u2010based, qPCR, and LAMP\u2013LFD detected 2, 41, and 37 positive samples, respectively. The LoDs were 10\n                      <jats:sup>2<\/jats:sup>\n                      and 10\n                      <jats:sup>1<\/jats:sup>\n                      \u2009CFU\/mL in fecal samples and 10\n                      <jats:sup>1<\/jats:sup>\n                      and 10\n                      <jats:sup>0<\/jats:sup>\n                      \u2009CFU\/mL in throat swab samples for\n                      <jats:italic>R. pneumotropicus<\/jats:italic>\n                      and\n                      <jats:italic>R. heylii<\/jats:italic>\n                      , respectively.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>This method provides an approach for monitoring the health status of laboratory mice by enabling the detection of these two target pathogens.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ame2.70214","type":"journal-article","created":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T05:22:12Z","timestamp":1780032132000},"page":"1395-1408","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Development of a loop\u2010mediated isothermal amplification\u2010lateral flow dipstick (\n                    <scp>LAMP<\/scp>\n                    \u2010\n                    <scp>LFD<\/scp>\n                    ) assay and on\u2010site rapid detection evaluation for\n                    <i>Rodentibacter heylii<\/i>\n                    and\n                    <i>Rodentibacter pneumotropicus<\/i>"],"prefix":"10.1002","volume":"9","author":[{"given":"Huiqiong","family":"Yan","sequence":"first","affiliation":[{"name":"Center of Laboratory Animal Hangzhou Medical College  Hangzhou Zhejiang China"},{"name":"Zhejiang Provincial Key Laboratory of Laboratory Animals and Safety Research 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