{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T16:20:11Z","timestamp":1787674811923,"version":"build-2784847793"},"reference-count":34,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T00:00:00Z","timestamp":1780617600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T00:00:00Z","timestamp":1780617600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Anim Models and Exp Med"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The development of immune reconstituted xenograft mouse models has addressed the limitations imposed by interspecies immunological differences in disease research. However, mouse models reconstituted with a sheep immune system remain largely unexplored.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>In this study, we successfully established a sheep splenocyte\u2010reconstituted mouse model (Sh\u2010Spl mice) by intravenously transplanting ovine splenocytes into severely immunodeficient NBSGW mice, and evaluated the efficiency of immune reconstitution.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Results showed that ovine\u2010derived CD45\n                      <jats:sup>+<\/jats:sup>\n                      , CD4\n                      <jats:sup>+<\/jats:sup>\n                      , and CD8\n                      <jats:sup>+<\/jats:sup>\n                      cells were detectable in the peripheral blood of Sh\u2010Spl mice for an extended period (up to 13\u2009weeks), and these cells were also distributed in the spleen, liver, and bone marrow. Furthermore, upon intraperitoneal infection with\n                      <jats:italic>Brucella melitensis<\/jats:italic>\n                      , Sh\u2010Spl mice exhibited characteristic pathological alterations and bacterial colonization, confirming the successful establishment of the infection model.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The sheep immune system\u2010reconstituted mouse model developed in this study, combined with subsequent pathogen challenge, provides a novel experimental platform for investigating the pathogenesis of sheep\u2010hosted pathogens and developing prevention and control strategies.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ame2.70217","type":"journal-article","created":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T08:46:13Z","timestamp":1780649173000},"page":"1602-1612","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Engraftment of sheep splenic lymphocytes into\n                    <scp>NBSGW<\/scp>\n                    mice and application in\n                    <i>Brucella<\/i>\n                    infection"],"prefix":"10.1002","volume":"9","author":[{"given":"Xiaowei","family":"Wang","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, Shanxi Agricultural University  Taigu Shanxi China"},{"name":"Institute of Infectious Diseases, Guangzhou Eighth People's Hospital Guangzhou Medical University  Guangzhou China"},{"name":"Guangzhou Key Laboratory of Clinical Pathogen Research for Infectious Diseases  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shicheng","family":"Wan","sequence":"additional","affiliation":[{"name":"Institute of Infectious Diseases, Guangzhou Eighth People's Hospital Guangzhou Medical University  Guangzhou China"},{"name":"Guangzhou Key Laboratory of Clinical Pathogen Research for Infectious Diseases  Guangzhou China"},{"name":"Shaanxi Centre of Stem Cells Engineering and Technology, College of Veterinary Medicine, Northwest A&amp;F University  Yangling Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanmin","family":"Guo","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Shanxi Agricultural University  Taigu Shanxi China"},{"name":"Institute of Infectious Diseases, Guangzhou Eighth People's Hospital Guangzhou Medical University  Guangzhou China"},{"name":"Guangzhou Key Laboratory of Clinical Pathogen Research for Infectious Diseases  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongyu","family":"Han","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Shanxi Agricultural University  Taigu Shanxi China"},{"name":"Institute of Infectious Diseases, Guangzhou Eighth People's Hospital Guangzhou Medical University  Guangzhou China"},{"name":"Guangzhou Key Laboratory of Clinical Pathogen Research for Infectious Diseases  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Luo","sequence":"additional","affiliation":[{"name":"Institute of Infectious Diseases, Guangzhou Eighth People's Hospital Guangzhou Medical University  Guangzhou China"},{"name":"Guangzhou Key Laboratory of Clinical Pathogen Research for Infectious Diseases  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongmei","family":"Jiang","sequence":"additional","affiliation":[{"name":"Institute of Infectious Diseases, Guangzhou Eighth People's Hospital Guangzhou Medical University  Guangzhou China"},{"name":"Guangzhou Key Laboratory of Clinical Pathogen Research for Infectious Diseases  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaorong","family":"Yang","sequence":"additional","affiliation":[{"name":"Institute of Infectious Diseases, Guangzhou Eighth People's Hospital Guangzhou Medical University  Guangzhou China"},{"name":"Guangzhou Key Laboratory of Clinical Pathogen Research for Infectious Diseases  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quanhai","family":"Pang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Shanxi Agricultural University  Taigu Shanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haisheng","family":"Yu","sequence":"additional","affiliation":[{"name":"Institute of Infectious Diseases, Guangzhou Eighth People's Hospital Guangzhou Medical University  Guangzhou China"},{"name":"Guangzhou Key Laboratory of Clinical Pathogen Research for Infectious Diseases  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9788-8592","authenticated-orcid":false,"given":"Jinlian","family":"Hua","sequence":"additional","affiliation":[{"name":"Shaanxi Centre of Stem Cells Engineering and Technology, College of Veterinary Medicine, Northwest A&amp;F University  Yangling Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,5]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-59745-170-3_21"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3089.1997.tb00188.x"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-2427(99)00065-3"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-021-01155-4"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-023-01906-5"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1080\/22221751.2025.2511134"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.24272\/j.issn.2095-8137.2025.155"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.34067\/KID.0000000000000227"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12015-018-9858-1"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202505635"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2025.105750"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stemcr.2014.12.005"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.73625"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/1297-9716-43-29"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2020.04.011"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2018.0217"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/ame2.12320"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/xen.12466"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2024.1365946"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-032713-120142"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00262-023-03465-9"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.642198"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/hem3.28"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1182\/bloodadvances.2023011371"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/cmi.2017.106"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imlet.2020.11.011"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcyt.2024.02.011"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.omtm.2019.10.017"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01109-10"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.05542-11"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41571-022-00721-2"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0000000000001283"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-025-04392-5"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1302561"}],"container-title":["Animal Models and Experimental Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ame2.70217","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/ame2.70217","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ame2.70217","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T15:26:08Z","timestamp":1787671568000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ame2.70217"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,5]]},"references-count":34,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["10.1002\/ame2.70217"],"URL":"https:\/\/doi.org\/10.1002\/ame2.70217","archive":["Portico"],"relation":{},"ISSN":["2576-2095","2576-2095"],"issn-type":[{"value":"2576-2095","type":"print"},{"value":"2576-2095","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,5]]},"assertion":[{"value":"2025-11-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-02","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-05","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}