{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T15:46:45Z","timestamp":1791733605237,"version":"4.3.4"},"reference-count":63,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,8,16]],"date-time":"2026-08-16T00:00:00Z","timestamp":1786838400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,8,16]],"date-time":"2026-08-16T00:00:00Z","timestamp":1786838400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Anim Models and Exp Med"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Knee osteoarthritis (KOA) is a chronic joint disorder. Current treatment options offer limited benefit. Adipose\u2010derived mesenchymal stem cells (ADSCs), known for their chondrogenic potential and anti\u2010inflammatory and immunomodulatory functions, have gained growing interest in regenerative therapy. Acupoint interventions are widely recognized for analgesic and anti\u2010inflammatory effects. Combining ADSCs with acupoint\u2010targeted delivery may represent a novel therapeutic approach. This study evaluated the therapeutic efficacy and underlying mechanisms of ADSC injection into the KI10 acupoint in mitigating KOA\u2010related cartilage damage.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>KOA models were established in male Sprague\u2013Dawley rats, followed by ADSC injection into the KI10 acupoint. Pain, motor function, and joint structural changes were assessed through ethological testing, imaging, histopathology, transmission electron microscopy, and molecular analyses. TMT\u2010based proteomics was used to identify mechanistic pathways, and the \u03b17nAChR antagonist methyllycaconitine citrate (MLA) was used to validate the pathways.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>ADSC injection into the KI10 acupoint significantly reduced pain and improved motor performance in KOA rats. Imaging and histological analysis revealed significant decreases in synovial inflammation and cartilage degeneration. Transmission electron microscopy confirmed diminished chondrocyte injury. Proteomic analysis indicated activation of the cholinergic anti\u2010inflammatory pathway, and MLA administration reversed these therapeutic benefits, verifying pathway involvement.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>ADSC injection into the KI10 acupoint effectively attenuates cartilage injury and inflammation in KOA by activating the PGE2\u2010mediated \u03b17nAChR\/NF\u2010\u03baB signaling pathway. This combined strategy harnesses the complementary advantages of stem cell therapy and acupoint\u2010targeted intervention, offering a promising therapeutic avenue for KOA.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ame2.70220","type":"journal-article","created":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T06:01:08Z","timestamp":1786946468000},"page":"1828-1848","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Adipose\u2010derived mesenchymal stem cell injection into the\n                    <scp>KI10<\/scp>\n                    acupoint mitigates cartilage damage in\n                    <scp>KOA<\/scp>\n                    rats through\n                    <scp>PGE2<\/scp>\n                    \u2010mediated\n                    <scp>\u03b17nAChR<\/scp>\n                    \/\n                    <scp>NF<\/scp>\n                    \u2010\n                    <scp>\u03baB<\/scp>\n            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