{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T18:58:58Z","timestamp":1785437938720,"version":"3.56.0"},"reference-count":30,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T00:00:00Z","timestamp":1780963200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T00:00:00Z","timestamp":1780963200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100010877","name":"Science, Technology and Innovation Commission of Shenzhen Municipality","doi-asserted-by":"publisher","award":["JCYJ20220530141615035"],"award-info":[{"award-number":["JCYJ20220530141615035"]}],"id":[{"id":"10.13039\/501100010877","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010877","name":"Science, Technology and Innovation Commission of Shenzhen Municipality","doi-asserted-by":"publisher","award":["20231120113324002"],"award-info":[{"award-number":["20231120113324002"]}],"id":[{"id":"10.13039\/501100010877","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Anim Models and Exp Med"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Psoriasis is an immune\u2010driven dermatosis marked by keratinocyte hyperproliferation. GS\u20109620, a TLR7 agonist, previously mitigated EV71\u2010triggered inflammation in mice; here we probe its anti\u2010psoriatic potential and mechanisms.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>IMQ\u2010induced psoriasis\u2010like mice were treated with GS\u20109620 or MTX; severity was tracked by PASI and histology. Skin\/spleen cytokines (IL\u20101\u03b2, IL\u20106, IL\u201018, HMGB1, TNF\u2010\u03b1) were quantified via ELISA; immune subsets were quantified by flow cytometry. Autophagy proteins (ATG5\/12\/16\u2009L1) and NLRP3 were assessed by IHC\/Western blot. In\u00a0vitro, M5\u2010stimulated primary keratinocytes were treated with GS\u20109620\u2009\u00b1\u2009autophagy modulators, followed by cytokine and protein analyses.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      GS\u20109620 markedly reduced erythema, scaling and epidermal thickness, lowered skin and systemic cytokines, and decreased splenic CD3\n                      <jats:sup>+<\/jats:sup>\n                      \/CD4\n                      <jats:sup>+<\/jats:sup>\n                      IL\u201017A\n                      <jats:sup>+<\/jats:sup>\n                      cells. It restored ATG5\/12\/16\u2009L1 expression while suppressing NLRP3 both in lesions and in M5\u2010stimulated keratinocytes, leading to diminished IL\u20101\u03b2, IL\u20106, IL\u201018, HMGB1 and TNF\u2010\u03b1 release.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>GS\u20109620 alleviates psoriasis by enhancing autophagy and dampening NLRP3\u2010mediated inflammation, offering a promising therapeutic avenue.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ame2.70222","type":"journal-article","created":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T04:16:17Z","timestamp":1781064977000},"page":"1364-1372","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>GS<\/scp>\n                    \u20109620 alleviates psoriasis\u2010like inflammation by regulating autophagy in keratinocytes"],"prefix":"10.1002","volume":"9","author":[{"given":"Yansi","family":"Lyu","sequence":"first","affiliation":[{"name":"Department of Dermatology, Shenzhen University General Hospital Shenzhen University  Shenzhen Guangdong P.\u00a0R.\u00a0China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Immunology, Shenzhen University Medical School  Shenzhen Guangdong P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pathology Affiliated Hospital of Hebei University  Baoding Hebei P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Immunology, Shenzhen University Medical School  Shenzhen Guangdong P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Wen","sequence":"additional","affiliation":[{"name":"Department of Immunology, Shenzhen University Medical School  Shenzhen Guangdong P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zigang","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Dermatology Hainan Hospital of PLA General Hospital  Sanya Hainan P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoqiang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Dermatology The First Hospital of Hebei Medical University  Shijiazhuang Hebei P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4516-3455","authenticated-orcid":false,"given":"Qian","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Dermatology Shenzhen Nanshan People's Hospital  Shenzhen Guangdong P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Si","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Immunology, Shenzhen University Medical School  Shenzhen Guangdong P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,9]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamadermatol.2018.5194"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20061475"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40659-020-00316-0"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clindermatol.2007.08.013"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2017.07.004"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2015.1071759"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2017.1402992"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2019.1671643"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.2012.01145.x"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/vetsci7030112"},{"key":"e_1_2_13_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2017.12.007"},{"key":"e_1_2_13_13_1","doi-asserted-by":"publisher","DOI":"10.3851\/IMP3359"},{"key":"e_1_2_13_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2018.02.002"},{"key":"e_1_2_13_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2004.04.012"},{"key":"e_1_2_13_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2009.241"},{"key":"e_1_2_13_17_1","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/7931031"},{"key":"e_1_2_13_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2018.09.048"},{"key":"e_1_2_13_19_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1300047"},{"key":"e_1_2_13_20_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0902464"},{"key":"e_1_2_13_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02166-16"},{"key":"e_1_2_13_22_1","doi-asserted-by":"publisher","DOI":"10.1080\/17460441.2018.1463214"},{"key":"e_1_2_13_23_1","doi-asserted-by":"publisher","DOI":"10.2147\/PTT.S327310"},{"key":"e_1_2_13_24_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-032713-120225"},{"key":"e_1_2_13_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2008.08769.x"},{"key":"e_1_2_13_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom12010060"},{"key":"e_1_2_13_27_1","doi-asserted-by":"publisher","DOI":"10.2741\/e679"},{"key":"e_1_2_13_28_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.00381"},{"key":"e_1_2_13_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1726"},{"key":"e_1_2_13_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature07383"},{"key":"e_1_2_13_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2006.02.004"}],"container-title":["Animal Models and Experimental Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ame2.70222","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/ame2.70222","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ame2.70222","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T18:16:31Z","timestamp":1785435391000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ame2.70222"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,9]]},"references-count":30,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2026,7]]}},"alternative-id":["10.1002\/ame2.70222"],"URL":"https:\/\/doi.org\/10.1002\/ame2.70222","archive":["Portico"],"relation":{},"ISSN":["2576-2095","2576-2095"],"issn-type":[{"value":"2576-2095","type":"print"},{"value":"2576-2095","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,9]]},"assertion":[{"value":"2025-12-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-14","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}