{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T18:59:11Z","timestamp":1785437951613,"version":"3.56.0"},"reference-count":29,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T00:00:00Z","timestamp":1782777600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T00:00:00Z","timestamp":1782777600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81925001"],"award-info":[{"award-number":["81925001"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82330070"],"award-info":[{"award-number":["82330070"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Anim Models and Exp Med"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Given the need to translate basic research into human therapies, the development and refinement of clinically relevant animal models for bronchiectasis are critically important. To date, there are no well\u2010established animal models for bronchiectasis. Thus, our aim was to develop a novel animal model that accurately recapitulates bronchiectasis\u2010like pathologies.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      To address this question, clinical strains of chronic\n                      <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                      (CPA) were embedded in agar beads in\u00a0vitro; then CPA\u2010loaded agar beads and papain were repeatedly instilled intratracheally in female C57BL\/6\u2009J mice. Experimental assessments included micro\u2010computed tomography (micro\u2010CT) imaging, histological analysis, immune cell infiltration, cytokines, and lung function parameter measurements to evaluate structural damage, immune responses, and lung function impairments in the mouse model.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>In this mouse model, we observed that lung micro\u2010CT imaging revealed significant bronchiectasis, with visible airways in the periphery, cylindrical airway expansion, and an airway\u2010to\u2010artery ratio &gt;\u20091. Histopathology highlighted immune cell infiltration around the trachea, including lymphocytes, neutrophils, and monocytes, along with Periodic acid\u2013Schiff staining\u2010positive hypermucinous secretion. Compared to controls, the bronchiectasis group exhibited elevated pro\u2010inflammatory cytokines in bronchoalveolar lavage fluid and worse lung function.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Our study presented a novel mouse model that better replicated the bronchiectasis\u2010like phenotype than the CPA airway infection model, showing the advantages of the \u201cCPA\u2010loaded agar beads and papain\u201d\u2013driven approach in optimizing the disease models. The model mimicked the progression of bronchiectasis closely and could be used for studies on disease pathogenesis as well as the evaluation of novel therapies in the near future.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ame2.70245","type":"journal-article","created":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T07:53:48Z","timestamp":1782892428000},"page":"1409-1419","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A novel mouse model of chronic\n                    <i>Pseudomonas aeruginosa<\/i>\n                    infection inducing bronchiectasis\u2010like phenotype"],"prefix":"10.1002","volume":"9","author":[{"given":"Heng","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine, Tongji Hospital, School of Medicine Tongji University  Shanghai China"},{"name":"Institute of Respiratory Medicine, Shanghai Pulmonary Hospital, School of 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