{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T08:44:20Z","timestamp":1792140260466,"version":"4.4.0"},"reference-count":45,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T00:00:00Z","timestamp":1787529600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T00:00:00Z","timestamp":1787529600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Anim Models and Exp Med"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Adriamycin (ADR)\u2010induced nephropathy is a widely used murine model of glomerular injury. Although strain\u2010dependent susceptibility is well recognized, variation among closely related substrains remains insufficiently explored.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We compared early responses to ADR between two BALB\/c substrains, BALB\/cAJcl (AJcl) and BALB\/cByJcl (ByJcl), focusing on baseline podocyte\u2010associated features and early glomerular transcriptional programs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Following ADR administration, ByJcl mice developed significantly greater albuminuria at Day 7 compared with AJcl mice. At Day 7, ByJcl mice also showed lower WT1\u2010positive nuclei counts and diminished nephrin immunoreactivity than AJcl mice; notably, these differences were already evident at baseline. Glomerulus\u2010enriched RNA sequencing showed that extracellular matrix organization and integrin signaling were already enriched in ByJcl at baseline and remained enriched at Day 5, when coordinated suppression of podocyte\u2010associated transcriptional programs was also evident.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Substrain\u2010dependent differences in baseline podocyte\u2010associated readouts and a pre\u2010existing cell\u2013matrix transcriptional state that persists after ADR are associated with divergent trajectories of ADR\u2010induced injury. Because WT1\u2010based counts cannot distinguish podocyte number from WT1 expression, these baseline differences are best described as an attenuated podocyte\u2010associated state rather than a definitive reduction in podocyte number. Careful documentation of substrain origin is critical for experimental reproducibility in nephropathy research.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ame2.70281","type":"journal-article","created":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T11:59:34Z","timestamp":1787572774000},"page":"1729-1742","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Baseline podocyte\u2010associated state and persistent cell\u2013matrix transcriptional programs are associated with\n                    <scp>BALB<\/scp>\n                    \/c substrain differences in adriamycin nephropathy"],"prefix":"10.1002","volume":"9","author":[{"given":"Ryuya","family":"Nakagawa","sequence":"first","affiliation":[{"name":"Laboratory of Laboratory Animal Science and Medicine, School of Veterinary Medicine Kitasato University  Towada Aomori 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