{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T09:59:43Z","timestamp":1790243983610,"version":"4.1.0"},"reference-count":41,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T00:00:00Z","timestamp":1790208000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T00:00:00Z","timestamp":1790208000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004479","name":"Natural Science Foundation of Jiangxi Province","doi-asserted-by":"publisher","award":["20242BAB20389"],"award-info":[{"award-number":["20242BAB20389"]}],"id":[{"id":"10.13039\/501100004479","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004479","name":"Natural Science Foundation of Jiangxi Province","doi-asserted-by":"publisher","award":["20244BCE52257"],"award-info":[{"award-number":["20244BCE52257"]}],"id":[{"id":"10.13039\/501100004479","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Anim Models and Exp Med"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>This study was designed to investigate whether chrysin, a natural flavone, alleviates experimental diabetic retinal disease and to define a microglia\u2010centered mechanism involving guanylate\u2010binding protein 3 (GBP3) and NLR family pyrin domain containing 3 (NLRP3)\u2013gasdermin D (GSDMD) pyroptosis signaling.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Streptozotocin (STZ)\u2010diabetic mice received vehicle or chrysin for 4\u2009weeks, followed by assessment of fasting blood glucose, serum caspase\u20101 and interleukin\u20101\u03b2 (IL\u20101\u03b2) levels, retinal histology, Evans blue leakage, and retinal immunofluorescence for glial fibrillary acidic protein (GFAP) and ionized calcium\u2010binding adaptor molecule 1 (IBA\u20101), with apoptosis measured by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL). In\u00a0vitro, BV2 microglia were exposed to high glucose with vehicle or chrysin. Cell viability (Cell Counting Kit\u20108), oxidative stress (reactive oxygen species and malondialdehyde), and pyroptosis\u2010associated markers were analyzed by Western blotting, reverse transcription quantitative polymerase chain reaction (RT\u2010qPCR), and immunofluorescence. RNA sequencing (RNA\u2010seq) profiled transcriptional responses, and small interfering RNA (siRNA) was used to knock down GBP3.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>In STZ\u2010diabetic mice, chrysin reduced fasting glucose and circulating caspase\u20101 and IL\u20101\u03b2, partially preserved retinal thickness, and decreased vascular leakage. Chrysin attenuated GFAP upregulation, reduced IBA\u20101 signals, and lowered TUNEL\u2010positive cells. In BV2 cells, chrysin improved viability, reduced oxidative stress, and suppressed NLRP3\u2013GSDMD activation. RNA\u2010seq highlighted chrysin\u2010responsive pyroptosis signatures and identified GBP3 as a regulated node. GBP3 knockdown reduced high\u2010glucose\u2010associated NLRP3 and Gsdmd expression and diminished GSDMD cleavage.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Chrysin protects the diabetic retina and suppresses microglial pyroptosis via a GBP3\u2010linked NLRP3\u2013GSDMD axis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ame2.70293","type":"journal-article","created":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T09:00:16Z","timestamp":1790240416000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Chrysin attenuates diabetes\u2010induced retinal injury by inhibiting microglial\n                    <scp>GBP3<\/scp>\n                    \/\n                    <scp>NLRP3<\/scp>\n                    \/\n                    <scp>GSDMD<\/scp>\n                    \u2010mediated pyroptosis"],"prefix":"10.1002","author":[{"given":"Qun","family":"Liu","sequence":"first","affiliation":[{"name":"School of Basic Medicine Nanchang Medical College  Nanchang PR China"},{"name":"Innovative Research Center for Major Disease Mechanisms and Intervention Nanchang Medical College  Nanchang PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Song\u2010min","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Basic Medicine Nanchang Medical College  Nanchang PR China"},{"name":"Innovative Research Center for Major Disease Mechanisms and Intervention Nanchang Medical College  Nanchang PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7823-4996","authenticated-orcid":false,"given":"Xuan","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Basic Medicine Nanchang Medical College  Nanchang PR China"},{"name":"Innovative Research Center for Major Disease 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