{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T16:36:50Z","timestamp":1793205410714,"version":"4.5.0"},"reference-count":34,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T00:00:00Z","timestamp":1792108800000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100021171","name":"Basic and Applied Basic Research Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2025A1515012787"],"award-info":[{"award-number":["2025A1515012787"]}],"id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Anim Models and Exp Med"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>ATP\u2010binding cassette transporter A1 (ABCA1) mediates cellular cholesterol efflux, yet its role in inflammatory bowel disease (IBD) remains incompletely understood. This study investigated the consequences of intestinal epithelial cholesterol accumulation\u2014primarily driven by ABCA1 deficiency\u2014on colonic inflammation and the underlying molecular mechanisms.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>ABCA1 knockout (KO) and wild\u2010type (WT) Syrian golden hamsters were fed a high\u2010cholesterol high\u2010fat diet for 12\u2009weeks. Colon specimens were analyzed for pathological changes, inflammatory markers, cholesterol accumulation, and gene expression profiles. Furthermore, in\u00a0vitro experiments were performed using the human colon epithelial cell line Caco\u20102.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>ABCA1 deficiency, in the context of a high\u2010cholesterol high\u2010fat diet, triggered significant colonic inflammation in KO hamsters, characterized by epithelial damage, inflammatory cell infiltration, and elevated pro\u2010inflammatory cytokine levels. These pathological changes were associated with impaired mucosal barrier integrity, a four\u2010fold increase in epithelial cholesterol accumulation, and reduced E\u2010cadherin, ZO\u20101 and occludin expression. Furthermore, ABCA1 deficiency promoted oxidative stress and induced marked remodeling of the gut microbiota.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our findings establish a pathogenic role for ABCA1 deficiency in driving colonic epithelial injury and inflammation, suggesting that ABCA1 may play an important role in the pathogenesis of IBD.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ame2.70301","type":"journal-article","created":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T07:48:02Z","timestamp":1792136882000},"page":"2063-2074","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>ABCA1<\/scp>\n                    deficiency\u2010induced cholesterol accumulation aggravates colonic epithelial injury in knockout hamsters"],"prefix":"10.1002","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-9771-2313","authenticated-orcid":false,"given":"Chang","family":"Liu","sequence":"first","affiliation":[{"name":"Guangdong Cardiovascular Institute, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences) Southern Medical University  Guangzhou China"},{"name":"Institute of Laboratory Animal Science, CAMS &amp; PUMC  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingjie","family":"Zheng","sequence":"additional","affiliation":[{"name":"Guangdong Province Key Laboratory of Large Animal Models for Biomedicine, South China Institute of Large Animal Models for Biomedicine, School of Pharmacy and Food Engineering Wuyi University  Jiangmen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sijing","family":"Shi","sequence":"additional","affiliation":[{"name":"Peking University Institute of Cardiovascular Sciences  Beijing 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