{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T11:12:25Z","timestamp":1791112345080,"version":"4.1.0"},"reference-count":38,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2002,5,31]],"date-time":"2002-05-31T00:00:00Z","timestamp":1022803200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"crossref","award":["SFB TR3"],"award-info":[{"award-number":["SFB TR3"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"crossref"}]},{"name":"BMBF","award":["NGFN"],"award-info":[{"award-number":["NGFN"]}]},{"DOI":"10.13039\/501100005972","name":"Deutsche Krebshilfe","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100005972","id-type":"DOI","asserted-by":"crossref"}]},{"name":"BONFOR program of the University of Bonn Medical Center"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of Neurology"],"published-print":{"date-parts":[[2002,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Focal cortical dysplasia (FCD) is characterized by a localized malformation of the neocortex and underlying white matter. Balloon cells, similar to those observed in tuberous sclerosis, are present in many cases (FCD<jats:sub>bc<\/jats:sub>). In these patients, a hyperintense funnel\u2010shaped subcortical lesion tapering toward the lateral ventricle was the characteristic finding on fluid\u2010attenuated inversion recovery magnetic resonance imaging scans. Surgical lesionectomy results in complete seizure relief. Although the pathogenesis of FCD<jats:sub>bc<\/jats:sub> remains uncertain, histopathological similarities indicate that FCD<jats:sub>bc<\/jats:sub> may be related pathogenetically to tuberous sclerosis. Here, we studied alterations of the <jats:italic>TSC1<\/jats:italic> and <jats:italic>TSC2<\/jats:italic> genes in a cohort of patients with chronic, focal epilepsy and histologically documented FCD<jats:sub>bc<\/jats:sub> (n = 48). DNA was obtained after microdissection and laser\u2010assisted isolation of balloon cells, dysplastic neurons, and nonlesional cells from adjacent normal brain tissue. Sequence alterations resulting in amino acid exchange of the <jats:italic>TSC1<\/jats:italic> gene product affecting exons 5 and 17 and silent base exchanges in exons 14 and 22 were increased in patients with FCD<jats:sub>bc<\/jats:sub> compared with 200 control individuals (exon 5, 2.3% FCD<jats:sub>bc<\/jats:sub> vs 0% C; exon 17, 35% FCD<jats:sub>bc<\/jats:sub> vs 1.0% C; exon 14, 37.8% FCD<jats:sub>bc<\/jats:sub> vs 15% C; exon 22, 45% FCD<jats:sub>bc<\/jats:sub> vs 23.8% C). Sequence alterations could be detected in FCD<jats:sub>bc<\/jats:sub> and in adjacent normal cells. In 24 patients, DNA was suitable to study loss of heterozygosity at the <jats:italic>TSC1<\/jats:italic> gene locus in microdissected FCD<jats:sub>bc<\/jats:sub> samples compared with control tissue. Eleven FCD<jats:sub>bc<\/jats:sub> cases exhibited loss of heterozygosity. In the <jats:italic>TSC2<\/jats:italic> gene, only silent polymorphisms were detected at similar frequencies as in controls. Our findings indicate that FCD<jats:sub>bc<\/jats:sub> constitutes a clinicopathological entity with distinct neuroradiological, neuropathological, and molecular genetic features. These data also suggest a role of the <jats:italic>TSC1<\/jats:italic> gene in the development of FCD<jats:sub>bc<\/jats:sub> and point toward a pathogenic relationship between FCD<jats:sub>bc<\/jats:sub> and the tuberous sclerosis complex.<\/jats:p>","DOI":"10.1002\/ana.10251","type":"journal-article","created":{"date-parts":[[2002,10,6]],"date-time":"2002-10-06T11:32:01Z","timestamp":1033903921000},"page":"29-37","source":"Crossref","is-referenced-by-count":131,"title":["Focal cortical dysplasia of Taylor's balloon cell type: Mutational analysis of the <i>TSC1<\/i> gene indicates a pathogenic relationship to tuberous sclerosis"],"prefix":"10.1002","volume":"52","author":[{"given":"Albert J.","family":"Becker","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Horst","family":"Urbach","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bj\u00f6rn","family":"Scheffler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Baden","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabine","family":"Normann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rainer","family":"Lahl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heinz W.","family":"Pannek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ingrid","family":"Tuxhorn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian E.","family":"Elger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johannes","family":"Schramm","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Otmar D.","family":"Wiestler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ingmar","family":"Bl\u00fcmcke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2002,6,18]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/1098-2779(2000)6:4<268::AID-MRDD6>3.0.CO;2-B"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.0305-1846.2001.00307.x"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1528-1157.1971.tb03913.x"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005072-199503000-00011"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1528-1157.2002.38201.x"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1042-3680(02)80002-X"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-1809.1994.tb01881.x"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1177\/088307389801301206"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.277.5327.805"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90618-Z"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(01)00333-6"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(01)00332-4"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80657-1"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/35010550"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/3.10.1833"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.2870130411"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/ajmg.1569"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1086\/302381"},{"key":"e_1_2_6_20_2","volume-title":"Molecular cloning","author":"Sambrook J","year":"1989"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.0305-1846.2001.00302.x"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004010050587"},{"key":"e_1_2_6_23_2","first-page":"204","article-title":"A device for processing large acrylamide gels","volume":"16","author":"Bender B","year":"1994","journal-title":"Biotechniques"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt0898-737"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/6.12.2155"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1136\/jmg.36.4.285"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1469-1809.1998.6260491.x"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-1004(1998)12:6<408::AID-HUMU7>3.0.CO;2-P"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-1004(1997)9:1<23::AID-HUMU4>3.0.CO;2-Q"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/5.2.249"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/3.10.1829"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng0294-193"},{"key":"e_1_2_6_33_2","first-page":"400","article-title":"Allelic loss is frequent in tuberous sclerosis kidney lesions but rare in brain lesions","volume":"59","author":"Henske EP","year":"1996","journal-title":"Am J Hum Genet"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1093\/jnen\/59.6.544"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01366952"},{"key":"e_1_2_6_36_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/7.6.1053"},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.10.6097"},{"key":"e_1_2_6_38_2","doi-asserted-by":"publisher","DOI":"10.1093\/jnen\/60.7.711"},{"key":"e_1_2_6_39_2","doi-asserted-by":"publisher","DOI":"10.1093\/jnen\/59.3.188"}],"container-title":["Annals of Neurology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fana.10251","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ana.10251","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,16]],"date-time":"2023-10-16T17:31:27Z","timestamp":1697477487000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ana.10251"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,6,18]]},"references-count":38,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2002,7]]}},"alternative-id":["10.1002\/ana.10251"],"URL":"https:\/\/doi.org\/10.1002\/ana.10251","archive":["Portico"],"relation":{},"ISSN":["0364-5134","1531-8249"],"issn-type":[{"value":"0364-5134","type":"print"},{"value":"1531-8249","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,6,18]]}}}