{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T22:42:24Z","timestamp":1792104144298,"version":"4.4.0"},"reference-count":41,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2002,7,17]],"date-time":"2002-07-17T00:00:00Z","timestamp":1026864000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of Neurology"],"published-print":{"date-parts":[[2002,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Persons affected with tuberous sclerosis complex (TSC) develop a wide range of neurological abnormalities including aberrant neuronal migration and seizures. In an effort to model TSC\u2010associated central nervous system abnormalities in mice, we generated two independent lines of astrocyte\u2010specific\n                    <jats:italic>Tsc1<\/jats:italic>\n                    conditional knockout mice by using the Cre\u2010LoxP system. Astrocyte\u2010specific\n                    <jats:italic>Tsc1<\/jats:italic>\n                    \u2010null mice exhibit electroencephalographically proven seizures after the first month of age and begin to die at 3 to 4 months.\n                    <jats:italic>Tsc1<\/jats:italic>\n                    \u2010null mice show significant increases in astrocyte numbers throughout the brain by 3 weeks of age and abnormal neuronal organization in the hippocampus between 3 and 5 weeks. Moreover, cultured\n                    <jats:italic>Tsc1<\/jats:italic>\n                    \u2010null astrocytes behave similar to wild\u2010type astrocytes during log phase growth but demonstrate increased saturation density associated with reduced p27\n                    <jats:sup>Kip1<\/jats:sup>\n                    expression. Collectively, our results demonstrate that astrocyte\u2010specific disruption of\n                    <jats:italic>Tsc1<\/jats:italic>\n                    in mice provides a context\u2010dependent growth advantage for astrocytes that results in abnormalities in neuronal organization and epilepsy.\n                  <\/jats:p>","DOI":"10.1002\/ana.10283","type":"journal-article","created":{"date-parts":[[2002,10,8]],"date-time":"2002-10-08T19:00:24Z","timestamp":1034103624000},"page":"285-296","source":"Crossref","is-referenced-by-count":338,"title":["Astrocyte\u2010specific\n                    <i>TSC1<\/i>\n                    conditional knockout mice exhibit abnormal neuronal organization and seizures"],"prefix":"10.1002","volume":"52","author":[{"given":"Erik J.","family":"Uhlmann","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Wong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca L.","family":"Baldwin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. Livia","family":"Bajenaru","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroaki","family":"Onda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David J.","family":"Kwiatkowski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelvin","family":"Yamada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David H.","family":"Gutmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2002,7,17]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1177\/088307389801301206"},{"key":"e_1_2_6_3_2","doi-asserted-by":"crossref","DOI":"10.1093\/oso\/9780195122107.001.0001","volume-title":"Tuberous sclerosis","author":"Gomez 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