{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T15:27:56Z","timestamp":1791041276277,"version":"4.1.0"},"reference-count":22,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2002,11,25]],"date-time":"2002-11-25T00:00:00Z","timestamp":1038182400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of Neurology"],"published-print":{"date-parts":[[2003,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The aim of this study was to clarify whether autoimmunity against P\/Q\u2010type voltage\u2010gated calcium channels (VGCCs) in the cerebellum was associated with the pathogenesis of paraneoplastic cerebellar degeneration (PCD) with Lambert\u2010Eaton myasthenic syndrome (LEMS). We used human autopsy cerebellar tissues from three PCD\u2010LEMS patients and six other disease patients including one with LEMS as the controls. We compared cerebellar P\/Q\u2010type VGCC in these patients and controls for the amount and ratio of autoantibody\u2010channel complex using an <jats:sup>125<\/jats:sup>I\u2010\u03c9\u2010conotoxin MVIIC\u2010binding assay with Scatchard analysis, and their distribution using autoradiography. The quantity of cerebellar P\/Q\u2010type VGCC measured by Scatchard analysis were reduced in PCD\u2010LEMS patients (63.0 \u00b1 7.0fmol\/mg, n = 3), compared with the controls (297.8 \u00b1 38.9fmol\/mg, n = 6). The ratio of autoantibody\u2010VGCC complexes to total P\/Q\u2010type VGCCs measured by immunoprecipitation assay were increased in PCD\u2010LEMS patients. We analysed cerebellar specimens by autoradiography using <jats:sup>125<\/jats:sup>I\u2010\u03c9\u2010conotoxin MVIIC, which specifically binds to P\/Q\u2010type VGCCs. In PCD\u2010LEMS cerebellum, the toxin binding sites of P\/Q\u2010type VGCCs were markedly reduced compared with controls, especially in the molecular layer, which is the richest area of P\/Q\u2010type VGCCs in the normal cerebellum. This suggests that P\/Q\u2010type VGCCs of the cerebellar molecular layer is the immunological target in developing PCD\u2010LEMS. <\/jats:p>","DOI":"10.1002\/ana.10392","type":"journal-article","created":{"date-parts":[[2003,1,29]],"date-time":"2003-01-29T15:35:29Z","timestamp":1043854529000},"page":"21-28","source":"Crossref","is-referenced-by-count":119,"title":["Reduction of P\/Q\u2010type calcium channels in the postmortem cerebellum of paraneoplastic cerebellar degeneration with Lambert\u2010Eaton myasthenic syndrome"],"prefix":"10.1002","volume":"53","author":[{"given":"Taku","family":"Fukuda","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masakatsu","family":"Motomura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoko","family":"Nakao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hirokazu","family":"Shiraishi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshiro","family":"Yoshimura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keisuke","family":"Iwanaga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mitsuhiro","family":"Tsujihata","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hirotoshi","family":"Dosaka\u2010Akita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katsumi","family":"Eguchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2002,11,25]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1136\/jnnp.58.1.85"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199506013322203"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.14.8328"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-510X(97)00296-7"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-5728(99)00210-6"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.1957.02970480021005"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.23.7.764"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.42.10.1944"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/120.8.1279"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.26.15245"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1096\/fj.00-0562fje"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/8070"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004150050303"},{"key":"e_1_2_6_15_2","first-page":"1237","article-title":"The Lambert\u2010Eaton myasthenic syndrome: a study of 110 Japanese cases","volume":"39","author":"Motomura M","year":"1999","journal-title":"Rinsho Shinkeigaku"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00418a025"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(91)90172-K"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(94)90774-9"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(94)90398-0"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.15-10-06403.1995"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.42.1.218"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200001063420104"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1001\/archneur.58.2.225"}],"container-title":["Annals of Neurology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fana.10392","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ana.10392","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,13]],"date-time":"2023-10-13T10:35:23Z","timestamp":1697193323000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ana.10392"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,11,25]]},"references-count":22,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2003,1]]}},"alternative-id":["10.1002\/ana.10392"],"URL":"https:\/\/doi.org\/10.1002\/ana.10392","archive":["Portico"],"relation":{},"ISSN":["0364-5134","1531-8249"],"issn-type":[{"value":"0364-5134","type":"print"},{"value":"1531-8249","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,11,25]]}}}