{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T11:12:24Z","timestamp":1791112344706,"version":"4.1.0"},"reference-count":20,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2003,6,30]],"date-time":"2003-06-30T00:00:00Z","timestamp":1056931200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of Neurology"],"published-print":{"date-parts":[[2003,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Excessive astrocytosis in cortical tubers in tuberous sclerosis complex (TSC) suggests that astrocytes may be important for epileptogenesis in TSC. We previously demonstrated that astrocyte\u2010specific<jats:italic>Tsc1<\/jats:italic>gene inactivation in mice (<jats:italic>Tsc1<\/jats:italic>cKO mice) results in progressive epilepsy. Here, we report that glutamate transporter expression and function is impaired in<jats:italic>Tsc1<\/jats:italic>cKO astrocytes.<jats:italic>Tsc1<\/jats:italic>cKO mice exhibit decreased GLT\u20101 and GLAST protein expression. Electrophysiological assays demonstrate a functional decrease in glutamate transport currents of<jats:italic>Tsc1<\/jats:italic>cKO astrocytes in hippocampal slices and astrocyte cultures. These findings suggest that<jats:italic>Tsc1<\/jats:italic>inactivation in astrocytes causes dysfunctional glutamate homeostasis, leading to seizure development in TSC. Ann Neurol 2003<\/jats:p>","DOI":"10.1002\/ana.10648","type":"journal-article","created":{"date-parts":[[2003,7,28]],"date-time":"2003-07-28T11:14:16Z","timestamp":1059390856000},"page":"251-256","source":"Crossref","is-referenced-by-count":180,"title":["Impaired glial glutamate transport in a mouse tuberous sclerosis epilepsy model"],"prefix":"10.1002","volume":"54","author":[{"given":"Michael","family":"Wong","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin C.","family":"Ess","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erik J.","family":"Uhlmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura A.","family":"Jansen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter B.","family":"Crino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven","family":"Mennerick","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelvin A.","family":"Yamada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David H.","family":"Gutmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2003,7,25]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"crossref","DOI":"10.1093\/oso\/9780195122107.001.0001","volume-title":"Tuberous sclerosis","author":"Gomez MR","year":"1999"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/ana.10283"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.38.6.920"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(93)90754-5"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.276.5319.1699"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(99)01945-9"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3940(00)01284-2"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-328X(97)00361-6"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-328X(98)00349-0"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.1997.68041564.x"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.2001.00612.x"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.0022-3042.2002.00768.x"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(99)03003-7"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.14-09-05559.1994"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(94)90038-8"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1001\/archneur.59.10.1524"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.298.5593.556"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/368059a0"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.18-12-04490.1998"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80086-0"}],"container-title":["Annals of Neurology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fana.10648","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ana.10648","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,10]],"date-time":"2024-01-10T10:12:49Z","timestamp":1704881569000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ana.10648"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,7,25]]},"references-count":20,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2003,8]]}},"alternative-id":["10.1002\/ana.10648"],"URL":"https:\/\/doi.org\/10.1002\/ana.10648","archive":["Portico"],"relation":{},"ISSN":["0364-5134","1531-8249"],"issn-type":[{"value":"0364-5134","type":"print"},{"value":"1531-8249","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,7,25]]}}}