{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T23:31:02Z","timestamp":1791415862460,"version":"4.3.3"},"reference-count":37,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2004,9,23]],"date-time":"2004-09-23T00:00:00Z","timestamp":1095897600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of Neurology"],"published-print":{"date-parts":[[2004,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Impairment of mitochondrial energy metabolism has been associated with a wide range of human disorders. Large\u2010scale partial deletions of mitochondrial DNA (mtDNA) cause sporadic Kearns\u2013Sayre syndrome, a fatal multisystem disorder, in which the majority of mtDNAs in affected tissues have deletions (\u0394\u2010mtDNAs). Since most mtDNA\u2010related diseases, including Kearns\u2013Sayre syndrome, are recessive, only a few wild\u2010type mtDNAs can compensate for the deleterious effects of many \u0394\u2010mtDNAs. We have developed a pharmacological approach to reduce the proportion of \u0394\u2010mtDNAs in vitro, in which we grow cells in medium containing ketone bodies, replacing glucose as the carbon source. Cells containing 100% \u0394\u2010mtDNA died after 5 days of treatment, whereas those containing 100% wild\u2010type mtDNA survived. Furthermore, in a cloned heteroplasmic cell line, the proportion of wild\u2010type mtDNA increased from 13% initially to approximately 22% after 5 days in ketogenic medium and was accompanied by a dramatic improvement in mitochondrial protein synthesis. We also present evidence that treatment with ketone bodies caused \u201cheteroplasmic shifting\u201d not only among cells (ie, intercellular selection) but also within cells (ie, intracellular selection). The demonstration that ketone bodies can distinguish between normal and respiratorily compromised cells points to the potential use of a ketogenic diet to treat patients with heteroplasmic mtDNA disorders. Ann Neurol 2004<\/jats:p>","DOI":"10.1002\/ana.20240","type":"journal-article","created":{"date-parts":[[2004,9,24]],"date-time":"2004-09-24T07:01:55Z","timestamp":1096009315000},"page":"662-669","source":"Crossref","is-referenced-by-count":140,"title":["Ketogenic treatment reduces deleted mitochondrial DNAs in cultured human cells"],"prefix":"10.1002","volume":"56","author":[{"given":"Sumana","family":"Santra","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert W.","family":"Gilkerson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mercy","family":"Davidson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric 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