{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T12:59:32Z","timestamp":1791032372189,"version":"4.1.0"},"reference-count":19,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2004,11,23]],"date-time":"2004-11-23T00:00:00Z","timestamp":1101168000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of Neurology"],"published-print":{"date-parts":[[2004,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We molecularly characterized human immunodeficiency virus type 1 (HIV\u20101) present in pure populations of astrocytes, macrophages, and multinucleated giant cells isolated using laser capture microdissection from brain tissue of two patients who died with HIV\u2010associated dementia. The V3 region of the HIV\u20101 envelope (<jats:italic>env<\/jats:italic>) gene was amplified from the pure\u2010cell populations, and multiple clones were sequenced. In both patients, the V3 <jats:italic>env<\/jats:italic> sequences were distinct in astrocytes compared with neighboring macrophages or multinucleated giant cells and were characteristic of CCR5\u2010using (R5) HIV\u20101. These results demonstrate cell\u2010specific compartmentalization of distinct R5\u2010like viral strains in the central nervous system microenvironment. Ann Neurol 2004<\/jats:p>","DOI":"10.1002\/ana.20304","type":"journal-article","created":{"date-parts":[[2004,11,23]],"date-time":"2004-11-23T23:49:08Z","timestamp":1101253748000},"page":"873-877","source":"Crossref","is-referenced-by-count":67,"title":["Astrocyte specific viral strains in HIV dementia"],"prefix":"10.1002","volume":"56","author":[{"given":"Katherine A.","family":"Thompson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melissa J.","family":"Churchill","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul R.","family":"Gorry","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jasminka","family":"Sterjovski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert B.","family":"Oelrichs","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven L.","family":"Wesselingh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catriona A.","family":"McLean","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,11,23]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"crossref","first-page":"488","DOI":"10.1128\/JVI.72.1.488-496.1998","article-title":"Analysis of the temporal relationship between human immunodeficiency virus type 1: quasispecies in sequential blood samples and various organs obtained at autopsy","volume":"72","author":"van't Wout AB","year":"1998","journal-title":"J Virol"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1089\/088922299310719"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1097\/00002030-199606000-00002"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/ana.1011"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004010051113"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410390606"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/35073667"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1750-3639.2003.tb00014.x"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.74.22.10581-10588.2000"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.74.3.1149-1157.2000"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.21.10073-10089.2001"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.2001.1087"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.2174\/1570162033485122"},{"key":"e_1_2_6_15_2","doi-asserted-by":"crossref","first-page":"1636","DOI":"10.4049\/jimmunol.163.3.1636","article-title":"Chemokine receptor expression and signaling in macaque and human fetal neurons and astrocytes: implications for the neuropathogenesis of AIDS","volume":"163","author":"Klein RS","year":"1999","journal-title":"J Immunol"},{"key":"e_1_2_6_16_2","first-page":"659","article-title":"Chemokine receptor expression on resident and inflammatory cells in the brain of macaques with simian immunodeficiency virus encephalitis","volume":"152","author":"Westmoreland SV","year":"1998","journal-title":"Am J Pathol"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1999.9998"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.78.8.4120-4133.2004"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1857969"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/glia.10175"}],"container-title":["Annals of Neurology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fana.20304","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ana.20304","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T15:37:32Z","timestamp":1696952252000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ana.20304"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,11,23]]},"references-count":19,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2004,12]]}},"alternative-id":["10.1002\/ana.20304"],"URL":"https:\/\/doi.org\/10.1002\/ana.20304","archive":["Portico"],"relation":{},"ISSN":["0364-5134","1531-8249"],"issn-type":[{"value":"0364-5134","type":"print"},{"value":"1531-8249","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,11,23]]}}}