{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T14:01:09Z","timestamp":1787320869599,"version":"build-2736575974"},"reference-count":35,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2012,4,20]],"date-time":"2012-04-20T00:00:00Z","timestamp":1334880000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of Neurology"],"published-print":{"date-parts":[[2012,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective:<\/jats:title>\n                    <jats:p>To use a new, unbiased biomarker discovery strategy to obtain and assess proteomic data from cerebrospinal fluid (CSF) of patients with multiple sclerosis (MS)\u2010related disorders.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>CSF protein profiles were analyzed from 107 patients with either MS\u2010related disorders (including relapsing remitting MS [RRMS], primary progressive MS [PPMS], anti\u2010aquaporin4 antibody seropositive\u2013neuromyelitis optica spectrum disorder [SP\u2010NMOSD], and seronegative\u2010NMOSD with long cord lesions on spinal magnetic resonance imaging [SN\u2010NMOSD]), amyotrophic lateral sclerosis (ALS), or other inflammatory neurological diseases (used as controls). CSF peptides\/proteins were purified with magnetic beads, and directly measured by matrix\u2010assisted laser desorption\/ionization time\u2010of\u2010flight mass spectrometry. The obtained spectra were analyzed with multivariate statistics and pattern matching algorithms. These analyses were replicated in an independent sample set of 84 patients composed of those with MS\u2010related disorders or with other neurological diseases (the second cohort).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>MS\u2010related disorders differed considerably in terms of CSF protein profiles. SP\u2010NMOSD and SN\u2010NMOSD, both of which fit within the NMO spectrum, were distinguishable from RRMS with high cross\u2010validation accuracy on a support vector machine classifier, especially in relapse phases. Some peaks derived from samples of relapsed SP\u2010NMOSD can discriminate RRMS with high area under curve scores (&gt;0.95) and this was reproduced on the second cohort. The similarity of proteomic patterns between selected neurological diseases were demonstrated by pattern matching analysis. To our surprise, the spectral differences between RRMS and PPMS were much larger than those of PPMS and ALS.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Interpretation:<\/jats:title>\n                    <jats:p>Our findings suggest that CSF proteomic pattern analysis can increase the accuracy of disease diagnosis of MS\u2010related disorders and will aid physicians in appropriate therapeutic decision\u2010making. ANN NEUROL 2012;<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ana.22633","type":"journal-article","created":{"date-parts":[[2011,9,12]],"date-time":"2011-09-12T12:41:31Z","timestamp":1315831291000},"page":"614-623","source":"Crossref","is-referenced-by-count":27,"title":["Proteomic pattern analysis discriminates among multiple sclerosis\u2013related disorders"],"prefix":"10.1002","volume":"71","author":[{"given":"Mika","family":"Komori","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yumiko","family":"Matsuyama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Nirasawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herbert","family":"Thiele","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Becker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theodore","family":"Alexandrov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takahiko","family":"Saida","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masami","family":"Tanaka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hidenori","family":"Matsuo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hidekazu","family":"Tomimoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryosuke","family":"Takahashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kei","family":"Tashiro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masaya","family":"Ikegawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takayuki","family":"Kondo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,4,20]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.23.021704.115707"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(07)70216-8"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1159\/000227277"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1212\/01.wnl.0000216139.44259.74"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)17551-X"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20050304"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1136\/jnnp.2006.114165"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1001\/archneur.63.7.964"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1001\/archneurol.2009.178"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1177\/1352458509104585"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(07)70243-0"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.0b013e3181c5b47f"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awp070"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1191\/1352458504ms1023oa"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1021\/pr0504788"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1021\/pr8010155"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200300715"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1177\/1352458507076406"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneuroim.2007.10.004"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/ana.20786"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2010.01.004"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awq035"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1136\/jnnp.2008.150698"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1212\/01.wnl.0000203129.82104.07"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.0030428"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.4172\/jpb.1000045"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1002\/ana.20703"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1177\/1352458507076976"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac800110w"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btn662"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2009.37"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2009.02788.x"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awq076"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awp106"},{"key":"e_1_2_6_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2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