{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T15:28:18Z","timestamp":1791300498767,"version":"4.1.0"},"reference-count":38,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2013,3,4]],"date-time":"2013-03-04T00:00:00Z","timestamp":1362355200000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of Neurology"],"published-print":{"date-parts":[[2013,3]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>Sporadic inclusion body myositis (sIBM) is an inflammatory myopathy characterized by both degenerative and autoimmune features. In contrast to other inflammatory myopathies, myositis\u2010specific autoantibodies had not been found in sIBM patients until recently. We used human skeletal muscle extracts as a source of antigens to detect autoantibodies in sIBM and to characterize the corresponding antigen.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Autoantibodies to skeletal muscle antigens were detected by immunoblotting. The target antigen was immunoaffinity\u2010purified from skeletal muscle extracts and characterized by mass spectrometry. A cDNA encoding this protein was cloned and expressed in vitro, and its recognition by patient sera was analyzed in an immunoprecipitation assay. Epitopes were mapped using microarrays of overlapping peptides.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>An M<jats:sub>r<\/jats:sub> 44,000 polypeptide (Mup44) was frequently targeted by sIBM autoantibodies. The target protein was purified, and subsequent mass spectrometry analysis revealed that Mup44 is the cytosolic 5\u2032\u2010nucleotidase 1A (cN1A). By immunoprecipitation of recombinant cN1A, high concentrations of anti\u2010Mup44 autoantibodies were detected in 33% of sIBM patient sera, whereas their prevalence in dermatomyositis, polymyositis, and other neuromuscular disorders appeared to be rare (4.2%, 4.5%, and 3.2%, respectively). Low concentrations of anti\u2010Mup44 antibodies were found in myositis as well as other neuromuscular disorders, but not in healthy controls. Three major autoepitope regions of cN1A were mapped by using microarrays containing a set of overlapping peptides covering the complete cN1A amino acid sequence.<\/jats:p><\/jats:sec><jats:sec><jats:title>Interpretation<\/jats:title><jats:p>Anti\u2010Mup44 autoantibodies, which are targeted to cN1A, represent the first serological biomarker for sIBM and may facilitate the diagnosis of this type of myositis. ANN NEUROL 2013;73:397\u2013407<\/jats:p><\/jats:sec>","DOI":"10.1002\/ana.23822","type":"journal-article","created":{"date-parts":[[2012,11,29]],"date-time":"2012-11-29T18:45:34Z","timestamp":1354214734000},"page":"397-407","source":"Crossref","is-referenced-by-count":225,"title":["Autoantibodies to cytosolic 5\u2032\u2010nucleotidase 1A in inclusion body myositis"],"prefix":"10.1002","volume":"73","author":[{"given":"Helma","family":"Pluk","sequence":"first","affiliation":[{"name":"Department of Biomolecular Chemistry Institute for Molecules and Materials and Nijmegen Center for Molecular Life Sciences Radboud University Nijmegen, Nijmegen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bas J. A.","family":"van Hoeve","sequence":"additional","affiliation":[{"name":"Department of Neurology, Center for Neuroscience, Donders Institute for Brain, Cognition, and Behavior, Radboud University Nijmegen Medical Center Nijmegen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sander H. J.","family":"van Dooren","sequence":"additional","affiliation":[{"name":"Department of Biomolecular Chemistry Institute for Molecules and Materials and Nijmegen Center for Molecular Life Sciences Radboud University Nijmegen, Nijmegen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judith","family":"Stammen\u2010Vogelzangs","sequence":"additional","affiliation":[{"name":"Department of Biomolecular Chemistry Institute for Molecules and Materials and Nijmegen Center for Molecular Life Sciences Radboud University Nijmegen, Nijmegen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annemarie","family":"van der Heijden","sequence":"additional","affiliation":[{"name":"Department of Biomolecular Chemistry Institute for Molecules and Materials and Nijmegen Center for Molecular Life Sciences Radboud University Nijmegen, Nijmegen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helenius J.","family":"Schelhaas","sequence":"additional","affiliation":[{"name":"Department of Neurology, Center for Neuroscience, Donders Institute for Brain, Cognition, and Behavior, Radboud University Nijmegen Medical Center Nijmegen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcel M.","family":"Verbeek","sequence":"additional","affiliation":[{"name":"Department of Neurology, Center for Neuroscience, Donders Institute for Brain, Cognition, and Behavior, Radboud University Nijmegen Medical Center Nijmegen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Umesh A.","family":"Badrising","sequence":"additional","affiliation":[{"name":"Department of Neurology Leiden University Medical Center Leiden the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Snjolaug","family":"Arnardottir","sequence":"additional","affiliation":[{"name":"Department of Clinical Neuroscience Karolinska Institute Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karina","family":"Gheorghe","sequence":"additional","affiliation":[{"name":"Rheumatology Unit Department of Medicine Karolinska University Hospital, Solna, Karolinska Institute Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ingrid E.","family":"Lundberg","sequence":"additional","affiliation":[{"name":"Rheumatology Unit Department of Medicine Karolinska University Hospital, Solna, Karolinska Institute Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wilbert C.","family":"Boelens","sequence":"additional","affiliation":[{"name":"Department of Biomolecular Chemistry Institute for Molecules and Materials and Nijmegen Center for Molecular Life Sciences Radboud University Nijmegen, Nijmegen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baziel G.","family":"van Engelen","sequence":"additional","affiliation":[{"name":"Department of Neurology, Center for Neuroscience, Donders Institute for Brain, Cognition, and Behavior, Radboud University Nijmegen Medical Center Nijmegen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ger J. M.","family":"Pruijn","sequence":"additional","affiliation":[{"name":"Department of Biomolecular Chemistry Institute for Molecules and Materials and Nijmegen Center for Molecular Life Sciences Radboud University Nijmegen, Nijmegen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,3,4]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/jnnp.2009.173823"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncpneuro0261"},{"key":"e_1_2_7_4_1","first-page":"161","article-title":"Sporadic inclusion body myositis: an unsolved mystery","volume":"34","author":"Machado P","year":"2009","journal-title":"Acta Reumatol Port"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(03)14368-1"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2010.2"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1517\/14656560902913815"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/ar2501"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2009.05119.x"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nmd.2007.11.001"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1097\/WCO.0b013e32833d3897"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1750-3639.2009.00290.x"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-010-0737-3"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-008-0471-2"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/ar2139"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1212\/01.wnl.0000192128.13875.1e"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11926-010-0102-5"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1097\/BOR.0b013e3283319265"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1212\/01.wnl.0000192129.65677.87"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.178.1.547"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1212\/01.wnl.0000187124.92826.20"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.59.8.1170"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1212\/01.WNL.0000148588.15052.4C"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneuroim.2012.05.003"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/PL00007850"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1136\/ard.60.2.116"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1212\/01.wnl.0000277527.69388.fe"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.autrev.2009.03.005"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410440325"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0020266"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-009-0554-8"},{"key":"e_1_2_7_32_1","first-page":"81","volume-title":"Diagnostic criteria for neuromuscular disorders","author":"Verschuuren JJ","year":"1997"},{"key":"e_1_2_7_33_1","first-page":"668","article-title":"Classification criteria for polymyositis and dermatomyositis","volume":"22","author":"Tanimoto K","year":"1995","journal-title":"J Rheumatol"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.10121"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.27665"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00415-005-0683-5"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410410116"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coph.2010.03.001"},{"key":"e_1_2_7_39_1","first-page":"251","article-title":"Interstitial 5\u2032\u2010nucleotidase stain for frozen biopsy specimens of skeletal muscle. A useful adjunct in the diagnosis of polymyositis","volume":"108","author":"El\u2010Shammaa NA","year":"1984","journal-title":"Arch Pathol Lab Med"}],"container-title":["Annals of Neurology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fana.23822","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ana.23822","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,22]],"date-time":"2023-11-22T15:19:26Z","timestamp":1700666366000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ana.23822"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,3]]},"references-count":38,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2013,3]]}},"alternative-id":["10.1002\/ana.23822"],"URL":"https:\/\/doi.org\/10.1002\/ana.23822","archive":["Portico"],"relation":{},"ISSN":["0364-5134","1531-8249"],"issn-type":[{"value":"0364-5134","type":"print"},{"value":"1531-8249","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,3]]}}}