{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T17:14:35Z","timestamp":1791566075213,"version":"4.3.4"},"reference-count":45,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,2,23]],"date-time":"2017-02-23T00:00:00Z","timestamp":1487808000000},"content-version":"vor","delay-in-days":22,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of Neurology"],"published-print":{"date-parts":[[2017,2]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>To report the identification of microtubule\u2010associated protein (MAP) 1B as the antigen of the previously described Purkinje cell cytoplasmic antibody type 2 (PCA\u20102) antibody, its frequency, and clinical, oncological, and serological associations.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Archival serum or cerebrospinal fluid (CSF) specimens were available from 96 of 118 consecutive PCA\u20102\u2010IgG\u2013seropositive patients identified during 1993\u20132016. The autoantigen, defined in mouse brain lysate by Western blot and mass spectrometry, was confirmed by dual immunohistochemical staining using commercial antibodies. The major antigenic region was defined by Western blot using recombinant protein fragments.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>IgG in 95 of 96 patients\u2019 serum or CSF (but in none of 98 healthy or disease control subjects\u2019 serum specimens) bound to recombinant MAP1B. A minority (17.5%) of patients\u2019 IgG also bound to MAP1A. PCA\u20102 was often accompanied by additional neural autoantibody markers of small\u2010cell carcinoma, including collapsin response\u2010mediated protein 5 (CRMP5) IgG (26%) or antineuronal nuclear antibody type 1 (ANNA\u20101) IgG (also known as anti\u2010Hu; 13%). Neurological manifestations in 95 patients were (in decreasing frequency): peripheral neuropathy, 53%; cerebellar ataxia, dysmetria, or dysarthria, 38%; and encephalopathy, 27%. Cancer (majority small\u2010cell lung carcinoma [SCLC]) was detected in 66 of 84 evaluated patients (79%). The MAP1B (PCA\u20102) autoantibody detection rate, among approximately 70,000 patients undergoing service neural autoantibody evaluation in 2015, was 0.024%, equaling amphiphysin IgG (0.026%) and more common than ANNA\u20102 (also known as anti\u2010Ri; 0.016%) and PCA\u2010Tr (also known as delta\/notch\u2010like epidermal growth factor\u2010related receptor [DNER]; 0.006%).<\/jats:p><\/jats:sec><jats:sec><jats:title>Interpretation<\/jats:title><jats:p>MAP1B, the PCA\u20102 autoantigen, represents a novel target in paraneoplastic neurological disorders and has high predictive value for SCLC. Its relatively high prevalence, compared with other recognized paraneoplastic neural autoantibodies, justifies its testing in comprehensive paraneoplastic neural autoantibody evaluation. Ann Neurol 2017;81:266\u2013277<\/jats:p><\/jats:sec>","DOI":"10.1002\/ana.24872","type":"journal-article","created":{"date-parts":[[2017,1,11]],"date-time":"2017-01-11T08:44:05Z","timestamp":1484124245000},"page":"266-277","source":"Crossref","is-referenced-by-count":89,"title":["Microtubule\u2010associated protein 1<scp>B<\/scp>: Novel paraneoplastic biomarker"],"prefix":"10.1002","volume":"81","author":[{"given":"Avi","family":"Gadoth","sequence":"first","affiliation":[{"name":"Departments of Laboratory Medicine and Pathology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas J.","family":"Kryzer","sequence":"additional","affiliation":[{"name":"Departments of Laboratory Medicine and Pathology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jim","family":"Fryer","sequence":"additional","affiliation":[{"name":"Departments of Laboratory Medicine and Pathology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"McKeon","sequence":"additional","affiliation":[{"name":"Departments of Laboratory Medicine and Pathology"},{"name":"Neurology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanda A.","family":"Lennon","sequence":"additional","affiliation":[{"name":"Departments of Laboratory Medicine and Pathology"},{"name":"Neurology"},{"name":"Immunology, Mayo Clinic Rochester MN"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sean J.","family":"Pittock","sequence":"additional","affiliation":[{"name":"Departments of Laboratory Medicine and 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