{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T14:50:02Z","timestamp":1787237402112,"version":"build-2736575974"},"reference-count":50,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,9,3]],"date-time":"2018-09-03T00:00:00Z","timestamp":1535932800000},"content-version":"vor","delay-in-days":33,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["16H02657"],"award-info":[{"award-number":["16H02657"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["16K09694"],"award-info":[{"award-number":["16K09694"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["15K19492"],"award-info":[{"award-number":["15K19492"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Annals of Neurology"],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To identify novel autoantibodies for neuropathic pain (NeP).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We screened autoantibodies that selectively bind to mouse unmyelinated C\u2010fiber type dorsal root ganglion (DRG) neurons using tissue\u2010based indirect immunofluorescence assays (IFA) with sera from 110 NeP patients with various inflammatory and allergic neurologic diseases or other neuropathies, and 50 controls without NeP including 20 healthy subjects and 30 patients with neurodegenerative diseases or systemic inflammatory diseases. IgG purified from IFA\u2010positive patients' sera was subjected to Western blotting (WB) and immunoprecipitation (IP) using mouse DRG lysates. Immunoprecipitates were analyzed by liquid chromatography tandem mass spectrometry (LC\u2010MS\/MS) to identify target autoantigens.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Antiunmyelinated C\u2010fiber type DRG neuron antibodies were more frequent in patients with NeP than non\u2010NeP subjects (10% vs 0%;\n                      <jats:italic>p<\/jats:italic>\n                      &lt; 0.05). These autoantibodies were all from the IgG2 subclass and colocalized mostly with isolectin B4\u2010 and P2X3\u2010positive pain\u2010conducting small neurons but not with S100\u03b2\u2010positive myelinated neurons. WB revealed a common immunoreactive band (approximately 220kDa). IP and LC\u2010MS\/MS studies identified plexin D1 as a target autoantigen. Immunoadsorption tests with recombinant human plexin D1 in IFA revealed that all 11 anti\u2013small DRG neuron antibody\u2010positive patients had anti\u2013plexin D1 antibodies. Application of anti\u2013plexin D1 antibody\u2010positive patient sera to cultured DRG neurons increased membrane permeability, leading to cellular swelling. NeP patients with anti\u2013plexin D1 antibodies commonly developed burning pain and current perception threshold abnormalities for C\u2010fibers. Main comorbidities were atopy and collagen\u2010vascular disease. Immunotherapies ameliorated NeP in 7 treated cases.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Interpretation<\/jats:title>\n                    <jats:p>Anti\u2013plexin D1 antibodies are a novel biomarker for immunotherapy\u2010responsive NeP. Ann Neurol 2018;84:208\u2013224<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ana.25279","type":"journal-article","created":{"date-parts":[[2018,7,17]],"date-time":"2018-07-17T02:32:47Z","timestamp":1531794767000},"page":"208-224","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["A Novel Autoantibody against Plexin\n                    <scp>D<\/scp>\n                    1 in Patients with Neuropathic Pain"],"prefix":"10.1002","volume":"84","author":[{"given":"Takayuki","family":"Fujii","sequence":"first","affiliation":[{"name":"Department of Neurology, Neurological Institute, Graduate School of Medical Sciences Kyushu University  Fukuoka"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryo","family":"Yamasaki","sequence":"additional","affiliation":[{"name":"Department of Neurology, Neurological Institute, Graduate School of Medical Sciences Kyushu University  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