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We investigated telomere length and DNA double\u2010strand breaks (histone variant p\u03b3\u2010H2AX) as predictive disease biomarkers in peripheral blood mononuclear cells (PBMC) from 25 premanifest subjects, 58 HD patients with similar CAG expansion in the huntingtin gene (<jats:italic>HTT<\/jats:italic>), and 44 healthy controls (HC). PBMC from the pre\u2010HD and HD groups showed shorter telomeres (<jats:italic>p<\/jats:italic> &lt; 0.0001) and a significant increase of p\u03b3\u2010H2AX compared to the controls (<jats:italic>p<\/jats:italic> &lt; 0.0001). The levels of p\u03b3\u2010H2AX correlated robustly with the presence of the mutated gene in pre\u2010HD and HD. The availability of a potentially reversible biomarker (p\u03b3\u2010H2AX) in the premanifest stage of HD, negligible in HC, provides a novel tool to monitor premanifest subjects and find patient\u2010specific drugs. 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University  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvio","family":"Peluso","sequence":"additional","affiliation":[{"name":"Department of Neurosciences University of Naples Federico II  Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristiana","family":"Borrelli","sequence":"additional","affiliation":[{"name":"Italian League for Research on Huntington and Related Diseases Foundation  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angelo","family":"Del Mondo","sequence":"additional","affiliation":[{"name":"Department of Biology University of Naples Federico II  Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Salvatore","sequence":"additional","affiliation":[{"name":"Department of Neurosciences University of Naples Federico II  Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luigi 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