{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T00:37:49Z","timestamp":1791333469217,"version":"4.3.1"},"reference-count":50,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,12,30]],"date-time":"2020-12-30T00:00:00Z","timestamp":1609286400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100008903","name":"Ministry of Health and Welfare","doi-asserted-by":"publisher","award":["MOHW107\u2010TDU\u2010B\u2010211\u2010123001"],"award-info":[{"award-number":["MOHW107\u2010TDU\u2010B\u2010211\u2010123001"]}],"id":[{"id":"10.13039\/100008903","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008903","name":"Ministry of Health and Welfare","doi-asserted-by":"publisher","award":["MOHW 108\u2010TDU\u2010B\u2010211\u201013"],"award-info":[{"award-number":["MOHW 108\u2010TDU\u2010B\u2010211\u201013"]}],"id":[{"id":"10.13039\/100008903","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology, Taiwan","doi-asserted-by":"publisher","award":["MOST\u2010107\u20102314\u2010B\u2010010\u2010021 & 108\u20102314\u2010B\u2010010 \u2010022 \u2010MY3 (to SPC) & MOST 108\u20102321\u2010B\u2010010\u2010014\u2010MY2, MOST 108\u20102321\u2010B\u2010010\u2010001\u2010, MOST 108\u20102314\u2010B\u2010010\u2010023\u2010MY3 (to SJW), and MOST 108\u20102633\u2010B\u2010009\u2010001 (to HDH and CHC)"],"award-info":[{"award-number":["MOST\u2010107\u20102314\u2010B\u2010010\u2010021 & 108\u20102314\u2010B\u2010010 \u2010022 \u2010MY3 (to SPC) & MOST 108\u20102321\u2010B\u2010010\u2010014\u2010MY2, MOST 108\u20102321\u2010B\u2010010\u2010001\u2010, MOST 108\u20102314\u2010B\u2010010\u2010023\u2010MY3 (to SJW), and MOST 108\u20102633\u2010B\u2010009\u2010001 (to HDH and CHC)"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011912","name":"Taipei Veterans General Hospital","doi-asserted-by":"publisher","award":["VGH\u2010106\u2010D9\u2010001\u2010MY2\u20102 (to SJW) & V108C\u2010066 (to SPC)"],"award-info":[{"award-number":["VGH\u2010106\u2010D9\u2010001\u2010MY2\u20102 (to SJW) & V108C\u2010066 (to SPC)"]}],"id":[{"id":"10.13039\/501100011912","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005382","name":"National Yang-Ming University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005382","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Annals of Neurology"],"published-print":{"date-parts":[[2021,3]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>The purpose of this study was to investigate the significance of circulating micro RNAs (miRNAs) in the pathogenesis of reversible cerebral vasoconstriction syndrome (RCVS).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We prospectively recruited 3 independent cohorts of patients with RCVS and age\u2010matched and sex\u2010matched controls in a single medical center. Next\u2010generation small RNA sequencing followed by quantitative polymerase chain reaction (PCR) was used to identify and validate differentially expressed miRNAs, which was cross\u2010validated in migraine patients in ictal stage or interictal stage. Computational analysis was used to predict the target genes of miRNAs, followed by in vitro functional analysis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>We identified a panel of miRNAs including miR\u2010130a\u20103p, miR\u2010130b\u20103p, let\u20107a\u20105p, let\u20107b\u20105p, and let\u20107f\u20105p that well differentiated patients with RCVS from controls (area under the receiver operating characteristics curve [AUC] was 0.906, 0.890, and 0.867 in the 3 cohorts, respectively). The abundance of let\u20107a\u20105p, let\u20107b\u20105p, and let\u20107f\u20105p, but not miR\u2010130a\u20103p nor miR\u2010130b\u20103p, was significantly higher in patients with ictal migraine compared with that of controls and patients with interictal migraine. Target prediction and pathway enrichment analysis suggested that the transforming growth factor\u2010\u03b2 signaling pathway and endothelin\u20101 responsible for vasomotor control might link these miRNAs to RCVS pathogenesis, which was confirmed in vitro by transfecting miRNAs mimics or incubating the patients\u2019 cerebrospinal fluid (CSF) in 3 different vascular endothelial cells. Moreover, miR\u2010130a\u20103p was associated with imaging\u2010proven disruption of the blood\u2010brain barrier (BBB) in patients with RCVS and its overexpression led to reduced transendothelial electrical resistance (ie, increased permeability) in in vitro human BBB model.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Interpretation<\/jats:title>\n                    <jats:p>We identified the circulating miRNA signatures associated with RCVS, which may be functionally linked to its headache, BBB integrity, and vasomotor function. ANN NEUROL 2021;89:459\u2013473<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ana.25965","type":"journal-article","created":{"date-parts":[[2020,12,13]],"date-time":"2020-12-13T02:28:28Z","timestamp":1607826508000},"page":"459-473","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":48,"title":["Circulating\n                    <scp>microRNAs<\/scp>\n                    Associated With Reversible Cerebral Vasoconstriction Syndrome"],"prefix":"10.1002","volume":"89","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3492-9902","authenticated-orcid":false,"given":"Shih\u2010Pin","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Neurology, Neurological Institute Taipei Veterans General Hospital  Taipei Taiwan"},{"name":"Division of Translational Research, Department of Medical Research Taipei Veterans General Hospital  Taipei Taiwan"},{"name":"Institute of Clinical Medicine, School of Medicine 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