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The \u03b1Syn\u2010SAA was also applied to frontal cortex and amygdala homogenates. Sensitivity and specificity were compared across distributions of \u03b1Syn pathology. Clinical data and co\u2010pathologies were compared across \u03b1Syn\u2010SAA positive and negative groups.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Fifty\u2010three individuals without and 66 with \u03b1Syn\u2010pathology (neocortical [n\u00a0=\u00a038], limbic [n\u00a0=\u00a07], and amygdala\u2010predominant [n\u00a0=\u00a021]) were included. There was a sensitivity of 97.8% and specificity of 98.1% of the \u03b1Syn\u2010SAA to identify patients with limbic\/neocortical pathology from antemortem CSF. Sensitivity to detect amygdala\u2010predominant pathology was only 14.3%. Postmortem CSF and brain tissue \u03b1Syn\u2010SAA analyses also showed higher assay positivity in samples from limbic\/neocortical cases.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Interpretation<\/jats:title>\n                    <jats:p>CSF \u03b1Syn\u2010SAA reliably identifies \u03b1Syn seeds in patients with diffuse \u03b1Syn pathology in the context of co\u2010pathology and non\u2010Lewy body disease (LBD) diagnoses. The analysis of brain homogenates suggests that pathological \u03b1Syn in the amygdala might differ from pathological \u03b1Syn in the frontal cortex. The \u03b1Syn\u2010SAA might facilitate the differential diagnosis of dementias with mixed pathologies. 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