{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T05:04:14Z","timestamp":1773291854849,"version":"3.50.1"},"reference-count":19,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2004,10,7]],"date-time":"2004-10-07T00:00:00Z","timestamp":1097107200000},"content-version":"vor","delay-in-days":9442,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of Neurology"],"published-print":{"date-parts":[[1978,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cerebrospinal fluid specimens from 31 patients with multiple sclerosis (MS) and 2 with progressive multifocal leukoencephalopathy (PML) were subjected to ultracentrifugation, and the resulting pellets were examined in an electron microscope. Cell types seen in the pellets included lymphocytes, occasional plasma cells, polymorphonuclear leukocytes, monocytes, eosinophils, lipid\u2010laden macrophages, and fibroblasts. The most interesting noncellular elements were extracellular myelin fragments, recognizable by their characteristic alternation of major dense lines and intraperiod lines. Myelin fragments were seen in the CSF from 7 of 9 patients with MS in exacerbation involving areas other than the optic nerve. These fragments were not observed in 4 specimens from patients with acute attacks manifested by optic neuritis. Myelin fragments were present in 1 of the 2 patients with PML. These observations indicate that a portion of the myelin destruction seen in MS and PML occurs extracellularly, with release of myelin fragments and degradation products into the CSF.<\/jats:p>","DOI":"10.1002\/ana.410040607","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T16:48:16Z","timestamp":1104598096000},"page":"515-523","source":"Crossref","is-referenced-by-count":27,"title":["Electron microscopic studies of cerebrospinal fluid sediment in demyelinating disease"],"prefix":"10.1002","volume":"4","author":[{"given":"Robert M.","family":"Herndon","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"Kasckow","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,10,7]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-66118-1_24"},{"key":"e_1_2_1_3_2","first-page":"225","volume-title":"The White Cell","author":"Cline MJ","year":"1975"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197612232952604"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1975.tb04329.x"},{"key":"e_1_2_1_6_2","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1093\/oxfordjournals.bmb.a071392","article-title":"Multiple sclerosis: a virus disease?","volume":"33","author":"Fraser KB","year":"1977","journal-title":"Br Med Bull"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.619457"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005072-197605000-00006"},{"key":"e_1_2_1_9_2","first-page":"320","article-title":"A method for the electron microscopic study of cerebrospinal fluid sediment","volume":"29","author":"Herndon RM","year":"1970","journal-title":"J Neuropathol Exp Neurol"},{"key":"e_1_2_1_10_2","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1001\/archinte.1972.03650020097018","article-title":"Central nervous system disease associated with Mycoplasma pneumoniae infections","volume":"130","author":"Hodges GR","year":"1972","journal-title":"Arch Intern Med"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410020515"},{"key":"e_1_2_1_12_2","first-page":"1","article-title":"The possible viral etiology of multiple sclerosis","volume":"13","author":"Johnson RT","year":"1975","journal-title":"Adv Neurol"},{"key":"e_1_2_1_13_2","first-page":"595","article-title":"Binding of 125I myelin basic protein by serum and cerebrospinal fluid","volume":"11","author":"Lennon V","year":"1972","journal-title":"Clin Exp Immunol"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2990.1977.tb00594.x"},{"key":"e_1_2_1_15_2","first-page":"856","article-title":"Radioimmunoassay of CSF for encephalitogenic basic protein: a diagnostic test for MS?","volume":"107","author":"McPherson TA","year":"1972","journal-title":"Can Med Assoc J"},{"key":"e_1_2_1_16_2","first-page":"369","article-title":"Chronic experimental allergic encephalomyelitis in inbred guinea pigs: an ultrastructural study","volume":"31","author":"Raine CS","year":"1974","journal-title":"Lab Invest"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1965.tb20235.x"},{"key":"e_1_2_1_18_2","volume-title":"Electron Microscopy of Human Blood Cells","author":"Tanaka Y","year":"1972"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197305242882106"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.27.10.911"}],"container-title":["Annals of Neurology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fana.410040607","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ana.410040607","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,2]],"date-time":"2023-11-02T07:57:17Z","timestamp":1698911837000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ana.410040607"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1978,12]]},"references-count":19,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1978,12]]}},"alternative-id":["10.1002\/ana.410040607"],"URL":"https:\/\/doi.org\/10.1002\/ana.410040607","archive":["Portico"],"relation":{},"ISSN":["0364-5134","1531-8249"],"issn-type":[{"value":"0364-5134","type":"print"},{"value":"1531-8249","type":"electronic"}],"subject":[],"published":{"date-parts":[[1978,12]]}}}