{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T23:06:45Z","timestamp":1791328005493,"version":"4.3.1"},"reference-count":40,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2004,10,8]],"date-time":"2004-10-08T00:00:00Z","timestamp":1097193600000},"content-version":"vor","delay-in-days":6643,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of Neurology"],"published-print":{"date-parts":[[1986,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Amyloid plaques are found in the brains of some patients with Creutzfeldt\u2010Jakob disease (CJD) and all patients with a related transmissible disorder, Gerstmann\u2010Str\u00e4ussler syndrome (GSS). In scrapie, a prion disease of animals, amyloid plaques have been shown to be composed of prion proteins (PrP), which form filaments of relatively uniform diameter. We report here that antisera raised against hamster scrapie PrP specifically stain amyloid plaques in the brains of both humans and rodents with CJD as well as a human subject with GSS. Earlier studies showed that these antibodies react with both rodent and human CJD PrP. The immunostained congophilic amyloid plaques in rodent brains measured 10 to 30 \u03bcm in diameter and exhibited a Maltese cross appearance. Limited proteolysis enchanced immunostaining of amyloid plaques in human brain sections from patients with CJD or GSS. Presumably proteolysis increases the exposure of those epitopes shared by human and rodent PrP. The differences in immunoreactivity between rodent and human amyloid plaques are consistent with other findings showing that cellular genes, not infectious purified prions, encode PrP.<\/jats:p>","DOI":"10.1002\/ana.410200205","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T17:34:20Z","timestamp":1104600860000},"page":"204-208","source":"Crossref","is-referenced-by-count":144,"title":["Amyloid plaques in Creutzfeldt\u2010Jakob disease stain with prion protein antibodies"],"prefix":"10.1002","volume":"20","author":[{"given":"Tetsuyuki","family":"Kitamoto","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Tateishi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takatoshi","family":"Tashima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iwao","family":"Takeshita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronald A.","family":"Barry","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen J.","family":"Dearmond","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stanley B.","family":"Prusiner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,10,8]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9975(85)90071-4"},{"key":"e_1_2_1_3_2","doi-asserted-by":"crossref","first-page":"603","DOI":"10.4049\/jimmunol.135.1.603","article-title":"Antibodies to the scrapie protein decorate prion rods","volume":"135","author":"Barry RA","year":"1985","journal-title":"J Immunol"},{"key":"e_1_2_1_4_2","first-page":"203","volume-title":"Slow, Latent and Temperate Virus Infections (NINDB Monograph 2)","author":"Beck E","year":"1965"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/310418a0"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.4.997"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198501103120202"},{"key":"e_1_2_1_8_2","doi-asserted-by":"crossref","first-page":"596","DOI":"10.1128\/jvi.53.2.596-606.1985","article-title":"Scrapie PrP 27\u201330 is a sialoglycoprotein","volume":"53","author":"Bolton DC","year":"1985","journal-title":"J Virol"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/315331a0"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00687490"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02866081"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(85)90076-5"},{"key":"e_1_2_1_13_2","first-page":"643","article-title":"Etude histochimique des plaques s\u00e9nile","volume":"27","author":"Divry P","year":"1927","journal-title":"J Belge Neurol Psychiatr"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-510X(69)90111-7"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.142303"},{"key":"e_1_2_1_16_2","first-page":"906","article-title":"\u00dcber ewin noch micht beschriebenes Reflex\u2014Ph\u00e4nomen bie einer Erkrankung des zerebellaren Systems","volume":"78","author":"Gerstmann J","year":"1928","journal-title":"Wien Med Wochenschr"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(84)80190-4"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.30.9.945"},{"key":"e_1_2_1_19_2","first-page":"231","article-title":"Systemic senile amyloid in senescence\u2010accelerated mice. A unique fibril protein demonstrated in tissues from various organs by the unlabeled immunoperoxidase method","volume":"48","author":"Higuchi K","year":"1983","journal-title":"Lab Invest"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02870932"},{"key":"e_1_2_1_21_2","volume-title":"Jakob\u2010Creutzfeldt Disease","author":"Kirschbaum WR","year":"1968"},{"key":"e_1_2_1_22_2","first-page":"799","volume":"8","author":"Klatzo I","year":"1959","journal-title":"Pathology of kuru. Lab Invest"},{"key":"e_1_2_1_23_2","first-page":"1","article-title":"Creutzfeldt\u2010Jakob disease: some unusual morphological features reminiscent of kuru","volume":"206","author":"Kr\u00fcck W","year":"1973","journal-title":"Z Neurol"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.12.4263"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/104.3.559"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.12.4245"},{"key":"e_1_2_1_27_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.6377496"},{"key":"e_1_2_1_28_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00691333"},{"key":"e_1_2_1_29_2","first-page":"246","article-title":"Alveolar rhabdomyosarcoma. Demonstration of the muscle type of intermediate filament protein, desmin, as a diagnostic aid","volume":"108","author":"Miettinen M","year":"1983","journal-title":"Am J Pathol"},{"key":"e_1_2_1_30_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00685286"},{"key":"e_1_2_1_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(85)90333-2"},{"key":"e_1_2_1_32_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.6801762"},{"key":"e_1_2_1_33_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198403083101021"},{"key":"e_1_2_1_34_2","first-page":"1","volume-title":"Advances in Virus Research","author":"Prusiner SB","year":"1984"},{"key":"e_1_2_1_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(84)90533-6"},{"key":"e_1_2_1_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(83)90168-X"},{"key":"e_1_2_1_37_2","doi-asserted-by":"publisher","DOI":"10.1177\/10.3.355"},{"key":"e_1_2_1_38_2","first-page":"104","volume-title":"The unlabeled antibody peroxidase\u2010antiperoxidase (PAP method). Immunocytochemistry","author":"Sternberger LA","year":"1979"},{"key":"e_1_2_1_39_2","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410150313"},{"key":"e_1_2_1_40_2","first-page":"951","volume-title":"Greenfield's Neuropathology","author":"Tomlinson BE","year":"1984"},{"key":"e_1_2_1_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(70)91095-0"}],"container-title":["Annals of Neurology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fana.410200205","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ana.410200205","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,20]],"date-time":"2023-10-20T22:55:37Z","timestamp":1697842537000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ana.410200205"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1986,8]]},"references-count":40,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1986,8]]}},"alternative-id":["10.1002\/ana.410200205"],"URL":"https:\/\/doi.org\/10.1002\/ana.410200205","archive":["Portico"],"relation":{},"ISSN":["0364-5134","1531-8249"],"issn-type":[{"value":"0364-5134","type":"print"},{"value":"1531-8249","type":"electronic"}],"subject":[],"published":{"date-parts":[[1986,8]]}}}