{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,3]],"date-time":"2025-10-03T09:16:50Z","timestamp":1759483010004},"reference-count":26,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2004,10,8]],"date-time":"2004-10-08T00:00:00Z","timestamp":1097193600000},"content-version":"vor","delay-in-days":6309,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of Neurology"],"published-print":{"date-parts":[[1987,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The presence of IgG antibodies reacting with purified and disrupted human T\u2010lymphotropic virus type I (HTLV\u2010I) was examined by an indirect enzyme\u2010linked immunosorbent assay (ELISA) in sera from 49 patients with multiple sclerosis (MS), 21 patients with aseptic meningoencephalitis (AM), 12 patients with Guillain\u2010Barr\u00e9 syndrome (GB), and 30 patients with tension headache (TH). This was also assessed in the concentrated cerebrospinal fluid (CSF) of most of these patients, as well as in sera of 60 blood donors (BD). Standardized amounts of serum IgG and CSF IgG were used in ELISA. For sera, higher reactivity with HTLV\u2010I was found in all four patient groups compared with the BD group, but no significant differences were observed among the four groups. There was higher reactivity with HTLV\u2010I in the CSF of patients with MS, AM, and GB compared to findings in patients with TH. Ten serum (2 MS, 3 GB, 3 TH, 2 BD) and 3 CSF (1 MS, 1 GB, 1 TH) specimens considered positive by ELISA for HTLV\u2010I were found negative on confirmatory Western blot analysis. We extended this study to analyze the in vitro production of anti\u2010HTLV\u2010I\u2013IgG antibodies by the 24\u2010hour cultivation of unstimulated lymphocytes from peripheral blood and CSF of 6 additional patients with MS directly in HTLV\u2010I antigen\u2013coated wells of microtiter plates. This was followed by determination of specific antibodies by ELISA in the same wells. No antibody production was measurable. Our data do not favor the hypothesis of an HTLV\u2010I\u2013related human retrovirus in the etiology of MS.<\/jats:p>","DOI":"10.1002\/ana.410220115","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T17:49:36Z","timestamp":1104601776000},"page":"67-71","source":"Crossref","is-referenced-by-count":20,"title":["Increased reactivity to HTLV\u2010I in inflammatory nervous system diseases"],"prefix":"10.1002","volume":"22","author":[{"given":"Francesco","family":"Lolli","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sten","family":"Fredrikson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Slavenka","family":"Kam\u2010Hansen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hans","family":"Link","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,10,8]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410090703"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.6189183"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910340212"},{"issue":"97","key":"e_1_2_1_5_2","first-page":"77","article-title":"Isolation of mononuclear cells and granulocytes from human blood","volume":"21","author":"Boyum A","year":"1968","journal-title":"Scand J Clin Lab Invest"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.253.23.3405"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.6342136"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3083.1986.tb02093.x"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.6200936"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(85)92734-5"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/322176a0"},{"key":"e_1_2_1_12_2","first-page":"1498","article-title":"Isolation of HTLV\u2010III from cerebrospinal fluid and neural tissue of patients with neurologic syndromes related to the acquired immunodeficiency syndrome","volume":"24","author":"Ho DD","year":"1985","journal-title":"N Engl J Med"},{"issue":"75","key":"e_1_2_1_13_2","first-page":"1","article-title":"Distribution and function of lymphocytes from the cerebrospinal fluid and blood in patients with multiple sclerosis","volume":"62","author":"Kam\u2010Hansen S","year":"1980","journal-title":"Acta Neurol Scand"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3083.1979.tb03271.x"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/322177a0"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/318154a0"},{"key":"e_1_2_1_17_2","first-page":"586","article-title":"Isolation of AIDS associated retroviruses from cerebrospinal fluid and brain of patients with neurological symptoms","volume":"2","author":"Levy JA","year":"1985","journal-title":"Lancet"},{"key":"e_1_2_1_18_2","doi-asserted-by":"crossref","first-page":"810","DOI":"10.1093\/clinchem\/29.5.810","article-title":"Utility of isoelectric focusing of cerebrospinal fluid and serum on agarose evaluated for neurological patients","volume":"299","author":"Link H","year":"1983","journal-title":"Clin Chem"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/7.1.89"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-5728(85)80027-8"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.77.12.7415"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.6089348"},{"key":"e_1_2_1_23_2","first-page":"361","article-title":"Application of the indirect enzymelinked immunosorbent assay microtest to the detection and surveillance of human T cell leukemia\u2010lymphoma virus","volume":"3","author":"Saxinger CW","year":"1983","journal-title":"Lab Invest"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1965.tb20235.x"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.3109\/00365517709091496"},{"key":"e_1_2_1_26_2","first-page":"55","article-title":"Enzyme immunoassay in diagnostic medicine. Theory and practice","volume":"53","author":"Voller A","year":"1976","journal-title":"Bull WHO"},{"key":"e_1_2_1_27_2","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1001\/jama.1985.03350260073029","article-title":"Screening test for HTLV\u2010III (AIDS agent) antibodies","volume":"23","author":"Weiss SH","year":"1985","journal-title":"JAMA"}],"container-title":["Annals of Neurology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fana.410220115","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ana.410220115","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,21]],"date-time":"2023-10-21T19:41:43Z","timestamp":1697917303000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ana.410220115"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1987,7]]},"references-count":26,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1987,7]]}},"alternative-id":["10.1002\/ana.410220115"],"URL":"https:\/\/doi.org\/10.1002\/ana.410220115","archive":["Portico"],"relation":{},"ISSN":["0364-5134","1531-8249"],"issn-type":[{"value":"0364-5134","type":"print"},{"value":"1531-8249","type":"electronic"}],"subject":[],"published":{"date-parts":[[1987,7]]}}}