{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T02:01:57Z","timestamp":1785722517172,"version":"3.56.0"},"reference-count":29,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2004,10,8]],"date-time":"2004-10-08T00:00:00Z","timestamp":1097193600000},"content-version":"vor","delay-in-days":5790,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of Neurology"],"published-print":{"date-parts":[[1988,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Human T\u2010cell lymphotropic virus type I (HTLV\u2010I)\u2010associated myelopathy (HAM) and tropical spastic paraparesis belong to a new group of neurological diseases associated with retroviral infection. An HTLV\u2010I\u2010like virus has recently been implicated in multiple sclerosis as well. We studied paired cerebrospinal fluid and serum specimens from HAM and multiple sclerosis patients by isoelectric focusing and an isoelectric focusing HTLV\u2010I p24 overlay technique to clarify the role of HTLV\u2010I in these diseases. We detected oligoclonal bands by isoelectric focusing with silver\u2010staining in cerebrospinal fluid, but not serum, from all 5 HAM and all 9 multiple sclerosis patients. An isoelectric focusing HTLV\u2010I p24 overlay technique demonstrated anti\u2010p24 antibody in HAM cerebrospinal fluid at a different pI distribution than that seen in paired serum, indicating local synthesis of anti\u2010p24 antibody within the central nervous system. Oligoclonal bands in HAM cerebrospinal fluid corresponded in pI distribution to anti\u2010p24 antibody activity, suggesting the presence of an ongoing HTLV\u2010I infection in the central nervous system. Multiple sclerosis patients had no evidence of anti\u2010HTLV\u2010I activity by p24 radioimmunoprecipitation assay, Western immunoblots, or isoelectric focusing HTLV\u2010I p24 overlay analysis. Our data support a role for HTLV\u2010I as an etiological agent in HAM, but not in multiple sclerosis.<\/jats:p>","DOI":"10.1002\/ana.410240606","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T14:06:32Z","timestamp":1104588392000},"page":"727-731","source":"Crossref","is-referenced-by-count":41,"title":["HTLV\u2010I\u2010associated myelopathy: Oligoclonal immunoglobulin G bands contain anti\u2010HTLV\u2010I p24 antibody"],"prefix":"10.1002","volume":"24","author":[{"given":"Luigi M. 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