{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T04:55:45Z","timestamp":1790225745869,"version":"4.1.0"},"reference-count":22,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2004,10,8]],"date-time":"2004-10-08T00:00:00Z","timestamp":1097193600000},"content-version":"vor","delay-in-days":5790,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of Neurology"],"published-print":{"date-parts":[[1988,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We identified and characterized the glucose transporter in the human cerebral cortex, cerebral microvessels, and choroid plexus by specific D\u2010glucose\u2010displaceable [<jats:sup>3<\/jats:sup>H]cytochalasin B binding. The binding was saturable, with a dissociation constant &lt; 1 \u03bcM. Maximal binding capacity was \u223d 7 pmol\/mg protein in the cerebral cortex, \u223d 42 pmol\/ mg protein in brain microvessels, and \u223d 27 pmol\/mg protein in the choroid plexus. Several hexoses displaced specific [3H]cytochalasin B binding to microvessels in a rank\u2010order that correlated well with their known ability to cross the blood\u2010brain barrier; the only exception was 2\u2010deoxy\u2010D\u2010glucose, which had much higher affinity for the glucose transporter than the natural substrate, D\u2010glucose. Irreversible photoaffinity labeling of the glucose transporter of microvessels with [3H]cytochalasin B, followed by solubilization and polyacrylamide gel electrophoresis, labeled a protein band with an average molecular weight of \u223d 55,000. Monoclonal and polyclonal antibodies specific to the human erythrocyte glucose transporter immunocytochemically stained brain blood vessels and the few trapped erythrocytes in situ, with minimal staining of the neuropil. In the choroid plexus, blood vessels did not stain, but the epithelium reacted positively. We conclude that human brain microvessels are richly endowed with a glucose transport moiety similar in molecular weight and antigenic characteristics to that of human erythrocytes and brain microvessels of other mammalian species.<\/jats:p>","DOI":"10.1002\/ana.410240610","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T14:06:32Z","timestamp":1104588392000},"page":"757-764","source":"Crossref","is-referenced-by-count":109,"title":["The glucose transporter of the human brain and blood\u2010brain barrier"],"prefix":"10.1002","volume":"24","author":[{"given":"Rajesh N.","family":"Kalaria","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen A.","family":"Gravina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James 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