{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T22:19:45Z","timestamp":1758925185729},"reference-count":33,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2004,10,8]],"date-time":"2004-10-08T00:00:00Z","timestamp":1097193600000},"content-version":"vor","delay-in-days":5608,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of Neurology"],"published-print":{"date-parts":[[1989,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In a patient with mitochondrial myopathy, the defect of cytochrome\n\u03f5 oxidase activity was restricted to some muscle fibers. To isolate cell lines with or without oxidase activity from a single muscle sample, primary cultured cells were transformed by replication origin\u2013defective simian virus 40, and then cloned. The clones were examined by cytochemical staining for cytochrome \u03f5 oxidase activity. Eight myogenic clones were completely devoid of activity, while the other myogenic and nonmyogenic clones were not. Deficiency of cytochrome \u03f5 oxidase was stable in culture for at least a year after serial passaging. The amount of mitochondrial DNA in cytochrome \u03f5 oxidase\u2014deficient cells was the same as in control cells, and no deletion in the mitochondrial DNA was detected. Protein synthesis in mitochondria of the subunits of cytochrome \u03f5 oxidase and subunit 6 of the ATP synthase complex was markedly decreased, whereas synthesis of the other subunits encoded by mitochondrial DNA was normal. These cloned cell lines provide an excellent system for clarifying the cause of mitochondrial myopathy and for investigating nuclear\u2010mitochondrial genetic interaction.<\/jats:p>","DOI":"10.1002\/ana.410250614","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T14:12:06Z","timestamp":1104588726000},"page":"615-621","source":"Crossref","is-referenced-by-count":11,"title":["Cytochrome <i>C<\/i> Oxidase\u2014deficient myogenic cell lines in mitochondrial myopathy"],"prefix":"10.1002","volume":"25","author":[{"given":"Hideo","family":"Shimoizumi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariko 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