{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T01:53:28Z","timestamp":1791165208172,"version":"4.1.0"},"reference-count":38,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2004,10,8]],"date-time":"2004-10-08T00:00:00Z","timestamp":1097193600000},"content-version":"vor","delay-in-days":4909,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of Neurology"],"published-print":{"date-parts":[[1991,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>T\u2010cell lines were expanded from muscle of 10 patients with polymyositis, 5 with inclusion body myositis, 5 with dermatomyositis, and 5 with other muscle diseases. All cell lines uniformly expressed T\u2010cell antigens, but not natural killer cell or B\u2010cell antigens. The proportion of helper (CD4<jats:sup>+<\/jats:sup>) and cytotoxic (CD8<jats:sup>+<\/jats:sup>) T cells in the expanded lines was variable and showed no correlation with the diagnosis. Sixteen cell lines (6 polymyositis, 4 inclusion body myositis, 5 dermatomyositis, 1 other muscle disease) consisted predominantly of CD8<jats:sup>+<\/jats:sup> T cells. None of these lines displayed natural killer\u2010like cytotoxicity but all were capable of lectin\u2010dependent cytotoxicity. Three of 6 polymyositis, 1 of 4 inclusion body myositis, and 1 of 5 dermatomyositis lines showed low but statistically significant cytotoxicity against autologous myotubes (6 to 27% specific <jats:sup>51<\/jats:sup>Cr release; effector\u2010target ratio, 20:1). The results demonstrate that functionally competent cytotoxic T cells can be expanded from muscle affected by inflammatory myopathies and are consistent with the hypothesis that some cytotoxic T cells recognize an autoantigen on myotubes. Further studies of this experimental system may define the molecular mechanism of T cell\u2010mediated muscle fiber injury and may help to identify the relevant antigens.<\/jats:p>","DOI":"10.1002\/ana.410290509","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T12:00:30Z","timestamp":1104580830000},"page":"498-507","source":"Crossref","is-referenced-by-count":80,"title":["Coculture with autologous myotubes of cytotoxic T cells isolated from muscle in inflammatory myopathies"],"prefix":"10.1002","volume":"29","author":[{"given":"Reinhard","family":"Hohlfeld","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew 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