{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T06:03:52Z","timestamp":1790661832297,"version":"4.1.0"},"reference-count":36,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2004,10,8]],"date-time":"2004-10-08T00:00:00Z","timestamp":1097193600000},"content-version":"vor","delay-in-days":4725,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of Neurology"],"published-print":{"date-parts":[[1991,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Both neural and nonneural human tissues from patients with or without Alzheimer's disease (AD) were surveyed to detect the presence of the \u03b2\u2010amyloid protein and its precursors. This was accomplished using polyclonal and monoclonal antibodies to epitopes in the 695 amino acid long \u03b2\u2010APP (i.e., \u03b2\u2010APP<jats:sub>695<\/jats:sub>), as well as in related \u03b2\u2010APPs. Immunoreactivity in \u03b2\u2010APP in brain was prominent in senile plaques, extraneuronal tangles, and neurons. Outside the brain, \u03b2\u2010APP staining was seen in neurons and satellite glial cells of the dorsal root, enteric and trigeminal gangila, the adeno\u2010and neurohypophysis, megakaryocytes, and adrenal gland in samples from patients with AD and those without AD. Western blots of neocortex revealed three major proteins with apparent molecular masses of 105, 115, and 125 kDa in the insoluble membrane\u2010associated fractions, while two broad bands with a molecular weight centered at about 100 and 120 kDa were detected in soluble fractions. In addition, the pituitary and adrenal glands as well as cardiac muscle revealed prominent immunobands in membrane\u2010associated fractions. Notably, other nonneural tissues were devoid of \u03b2\u2010APP immunoreactivity. Thus, the \u03b2\u2010APPs are detectable only in a limited number of nonneural tissues. Taken together, these data suggest that \u03b2\u2010APPs produced in the brain are sources of \u03b2\u2010APP peptides that accumulate as senile plaques in AD.<\/jats:p>","DOI":"10.1002\/ana.410300509","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T12:06:06Z","timestamp":1104581166000},"page":"686-693","source":"Crossref","is-referenced-by-count":141,"title":["Expression patterns of \u03b2\u2010amyloid precursor protein (\u03b2\u2010APP) in neural and nonneural human tissues from alzheimer's disease and control subjects"],"prefix":"10.1002","volume":"30","author":[{"given":"Hiroyuki","family":"Arai","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Virginia M.\u2010Y.","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meridith L.","family":"Messinger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barry D.","family":"Greenberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David E.","family":"Lowery","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John Q.","family":"Trojanowski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,10,8]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"167","article-title":"Vulnerability of the neuronal cytoskeleton in aging and Alzheimer's disease: widespread involvement of all three major filament systems","volume":"10","author":"Trojanowski JQ","year":"1990","journal-title":"Annu Rev Gerontol Geriatr"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.5.3.2001787"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.6.2249"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(90)90573-T"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410250307"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(83)91256-4"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.40.8.1302"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcla.1860040104"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-5728(84)80027-2"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1988.tb02822.x"},{"key":"e_1_2_1_12_2","first-page":"19","article-title":"Ultrastructural localization of the putative precursors of the A4 amyloid protein associated with Alzheimer's disease","volume":"137","author":"Catteruccia N","year":"1990","journal-title":"Am J Pathol"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(88)81038-X"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.15.6003"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-510X(90)90097-7"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2110384"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/341226a0"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.16.6338"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(90)90177-S"},{"key":"e_1_2_1_20_2","first-page":"897","volume-title":"Psychopharmacology: the third generation of progress","author":"Culter NR","year":"1987"},{"key":"e_1_2_1_21_2","doi-asserted-by":"crossref","unstructured":"LoweryDE PasternakJM Gonzalez\u2010De WhittPA et al. 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