{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T21:40:55Z","timestamp":1789940455834,"version":"4.0.1"},"reference-count":23,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2004,10,8]],"date-time":"2004-10-08T00:00:00Z","timestamp":1097193600000},"content-version":"vor","delay-in-days":4359,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of Neurology"],"published-print":{"date-parts":[[1992,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Recently, it has been shown that in patients with human T\u2010cell lymphotropic virus type I (HTLV\u2010I)\u2013associated neurological disease, high levels of HTLV\u2010I\u2013specific cytotoxic T lymphocytes (CTLs) could be detected in the peripheral blood. These CTLs predominantly recognized products of the pX region of HTLV\u2010I, had a CD8<jats:sup>+<\/jats:sup> phenotype, and were human leukocyte class I restricted. Moreover, these responses were not detected in asymptomatic, HTLV\u2010I\u2013seropositive individuals. This implied a role for these CTLs in the pathogenesis of the neurological disorder associated with HTLV\u2010I. We have extended these observations by demonstrating HTLV\u2010I\u2013specific CTLs directly from lymphocytes obtained from the cerebrospinal fluid of patients with HTLV\u2010I\u2013associated myelopathy\/tropical spastic parapare\u2010sis. Uncultured cerebrospinal fluid lymphocytes were used directly as effectors on a variety of targets expressing HTLV\u2010I. These cells were lysed in a virus\u2010specific and HLA class I\u2010restricted manner. Moreover, the cerebrospinal fluid lymphocytes were sorted into purified CD8<jats:sup>+<\/jats:sup> populations, cloned by limiting dilution, and assayed for CTL activity. An exceedingly high proportion of these resultant lines were shown to be cytolytic and precursor frequency analysis indicated that as many as 1 in 500 cells were HTLV\u2010I\u2013specific CTLs. The majority of these CTL lines recognized HTLV\u2010I gene products encoded within the pX region of HTLV\u2010I. The significance of these HTLV\u2010I\u2013specific CTLs in the central nervous system of patients with HTLV\u2010I\u2013associated neurological disease is discussed with regard to the potential role of CTLs in the pathogenesis of this disease.<\/jats:p>","DOI":"10.1002\/ana.410320508","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T12:17:32Z","timestamp":1104581852000},"page":"651-657","source":"Crossref","is-referenced-by-count":61,"title":["HTLV\u2010I\u2010specific cytotoxic T lymphocytes in the cerebrospinal fluid of patients with HTLV\u2010I\u2010associated neurological disease"],"prefix":"10.1002","volume":"32","author":[{"given":"Steven","family":"Jacobson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dale 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