{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T20:05:07Z","timestamp":1787169907434,"version":"build-2736575974"},"reference-count":31,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2004,10,8]],"date-time":"2004-10-08T00:00:00Z","timestamp":1097193600000},"content-version":"vor","delay-in-days":4298,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of Neurology"],"published-print":{"date-parts":[[1993,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Microphotometric enzyme assay was used to study cytochrome\n                    <jats:italic>c<\/jats:italic>\n                    oxidase activity in single human skeletal muscle fibers. The assay techniques combine the precise localization of enzyme activity provided by histochemical methodology with the precise quantitation of a sensitive assay system. Abnormalities of cytochrome\n                    <jats:italic>c<\/jats:italic>\n                    oxidase were investigated using microphotometric enzyme assay in 12 patients with Kearns\u2010Sayre syndrome, chronic progressive external ophthalmoplegia, or Leigh's syndrome. Control values were obtained using muscle biopsy specimens from 20 juvenile and 18 adult subjects with no evidence of neuromuscular disease. In the patients with Leigh's syndrome due to cytochrome\n                    <jats:italic>c<\/jats:italic>\n                    oxidase deficiency, the abnormality was found to be expressed uniformly throughout the muscle fiber population. In contrast, patients with Kearns\u2010Sayre syndrome or chronic progressive external ophthalmoplegia showed abnormal heterogeneity of cytochrome\n                    <jats:italic>c<\/jats:italic>\n                    oxidase activity. In many cases, extreme degrees of variability were seen, with fibers containing high activity adjacent to fibers with no detectable activity. Mitochondrial DNA analysis showed that most of the patients with Kearns\u2010Sayre syndrome and chronic progressive external ophthalmoplegia had major rearrangements of mitochondrial DNA. It was concluded that the extreme variability of cytochrome\n                    <jats:italic>c<\/jats:italic>\n                    oxidase activity detected using microphotometric enzyme assay was an indicator of a probable abnormality of mitochondrial DNA. Conversely, cytochrome\n                    <jats:italic>c<\/jats:italic>\n                    oxidase defects in muscle which show a homogeneous distribution are more likely to be associated with defects of the nuclear genome.\n                  <\/jats:p>","DOI":"10.1002\/ana.410330106","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T07:21:38Z","timestamp":1104564098000},"page":"28-35","source":"Crossref","is-referenced-by-count":41,"title":["Cytochrome\n                    <i>c<\/i>\n                    oxidase activity in single muscle fibers: Assay techniques and diagnostic applications"],"prefix":"10.1002","volume":"33","author":[{"given":"Margaret A.","family":"Johnson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurence A.","family":"Bindoff","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Douglass M.","family":"Turnbull","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,10,8]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-197711100-00005"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410140209"},{"key":"e_1_2_1_4_2","doi-asserted-by":"crossref","first-page":"850","DOI":"10.1542\/peds.60.6.850","article-title":"Leigh's encephalomyelopathy in a patient with cytochrome oxidase deficiency in muscle tissue","volume":"60","author":"Willems 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