{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T09:12:11Z","timestamp":1790759531871,"version":"4.1.0"},"reference-count":46,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2004,10,8]],"date-time":"2004-10-08T00:00:00Z","timestamp":1097193600000},"content-version":"vor","delay-in-days":4298,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of Neurology"],"published-print":{"date-parts":[[1993,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Lambert\u2010Eaton myasthenic syndrome is a paraneoplastic neuromuscular disorder in which an immune response directed against a small\u2010cell lung tumor crossreacts with antigens in the neuromuscular junction. To isolate and characterize the antigens, We screened a human fetal brain expression library with a high\u2010titer serum from a patient with Lambert\u2010Eaton myasthenic syndrome. This screening resulted in the isolation of a complementary DNA clone encoding an antigen we call myasthenic syndrome antigen B (MysB). Approximately 43% (3 of 7) of Lambert\u2010Eaton myasthenic syndrome sera specifically recognized MysB fusion protein, whereas none of 34 control sera did. The predicted amino acid sequence of this clone shows a high degree of homology to the b\u0303 subunit of calcium channel complexes. The MysB pre\u2010messenger RNA is alternatively spliced to yeild 3 forms of the protein differing in the domain between two highly conserved \u03b1\u2010helical segments.<\/jats:p>","DOI":"10.1002\/ana.410330126","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T12:21:38Z","timestamp":1104582098000},"page":"113-120","source":"Crossref","is-referenced-by-count":52,"title":["Cloning and characterization of a lambert\u2010eaton myasthenic syndrome antigen"],"prefix":"10.1002","volume":"33","author":[{"given":"Myrna R.","family":"Rosenfeld","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ellen","family":"Wong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josep","family":"Dalmau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geoff","family":"Manley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jerome B.","family":"Ponser","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emanuele","family":"Sher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henry M.","family":"Furneaux","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,10,8]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"187","volume-title":"Immunologic mechanisms in neurologic and psychiatric disease","author":"Posner JB","year":"1990"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(81)90474-8"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/mus.880080213"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2447652"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(53)91358-0"},{"key":"e_1_2_1_7_2","first-page":"612","article-title":"Defect of neuromuscular conduction associated with malignant neoplasms","volume":"187","author":"Lambert EH","year":"1956","journal-title":"Am J Physiol"},{"key":"e_1_2_1_8_2","first-page":"689","article-title":"Detailed analysis of neuromuscular transmission in a patient with the myasthenic syndrome sometimes associated with bronchogenic carcinoma","volume":"43","author":"Elmqvist D","year":"1968","journal-title":"Mayo Clin Proc"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/mus.880050905"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.32.9.1061"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.27.2.141"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/317737a0"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(89)90893-3"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(91)90376-Y"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(91)90981-Z"},{"key":"e_1_2_1_16_2","first-page":"4719","article-title":"Antagonism of voltage\u2010gated calcium channels in small cell carcinomas of patients with and without Lambert\u2010Eaton myasthenic syndrome by autoantibodies, \u03c9\u2010conotoxin and adenosine","volume":"48","author":"De Aizpurua HJ","year":"1988","journal-title":"Cancer Res"},{"key":"e_1_2_1_17_2","first-page":"97","article-title":"Lambert\u2010Eaton syndrome antibodies: reaction with membranes from a small cell lung cancer xenograft","volume":"18","author":"Chester KA","year":"1988","journal-title":"J Immunol"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410290313"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2549640"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(91)81296-K"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(92)90109-Q"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(83)90418-9"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.9.4350"},{"key":"e_1_2_1_24_2","volume-title":"Molecular cloning: a laboratory manual, ed 2","author":"Sambrook J","year":"1989"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1708916"},{"key":"e_1_2_1_27_2","doi-asserted-by":"crossref","first-page":"18914","DOI":"10.1016\/S0021-9258(18)55150-4","article-title":"A monoclonal antibody to the \u03b2\u2010subunit of the skeletal muscle dihydropyridine receptor immunoprecipitates the brain \u03c9\u2010conotoxin GVIA receptor","volume":"266","author":"Sakamoto J","year":"1991","journal-title":"J Biol Chem"},{"key":"e_1_2_1_28_2","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/111.3.577"},{"key":"e_1_2_1_29_2","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410250310"},{"key":"e_1_2_1_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0025-6196(12)65705-X"},{"key":"e_1_2_1_31_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.8.3625"},{"key":"e_1_2_1_32_2","first-page":"30","volume-title":"The biology and medicine of signal transduction","author":"Catterall WA","year":"1990"},{"key":"e_1_2_1_33_2","doi-asserted-by":"publisher","DOI":"10.1038\/328313a0"},{"key":"e_1_2_1_34_2","doi-asserted-by":"publisher","DOI":"10.1038\/352159a0"},{"key":"e_1_2_1_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/350398a0"},{"key":"e_1_2_1_36_2","doi-asserted-by":"crossref","unstructured":"PassafaroM ClementiF SherE. Metabolism of \u03c9\u2010conotoxin\u2010sensitive voltage\u2010operated calcium channels in human neuroblastoma cells: modulation by cell differentiation and antichannel antibodies.J Neurosci1993(in press)","DOI":"10.1523\/JNEUROSCI.12-09-03372.1992"},{"key":"e_1_2_1_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/0166-2236(88)90193-2"},{"key":"e_1_2_1_38_2","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410170610"},{"key":"e_1_2_1_39_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910300404"},{"key":"e_1_2_1_40_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.8.3439"},{"key":"e_1_2_1_41_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.8.2873"},{"key":"e_1_2_1_42_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.8.3451"},{"key":"e_1_2_1_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(91)90184-Z"},{"key":"e_1_2_1_44_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199006283222604"},{"key":"e_1_2_1_45_2","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.41.11.1757"},{"key":"e_1_2_1_46_2","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410290303"},{"key":"e_1_2_1_47_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005792-199203000-00001"}],"container-title":["Annals of Neurology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fana.410330126","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ana.410330126","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,17]],"date-time":"2024-01-17T21:32:58Z","timestamp":1705527178000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ana.410330126"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,1]]},"references-count":46,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1993,1]]}},"alternative-id":["10.1002\/ana.410330126"],"URL":"https:\/\/doi.org\/10.1002\/ana.410330126","archive":["Portico"],"relation":{},"ISSN":["0364-5134","1531-8249"],"issn-type":[{"value":"0364-5134","type":"print"},{"value":"1531-8249","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,1]]}}}