{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T00:08:36Z","timestamp":1758845316718},"reference-count":15,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2004,10,8]],"date-time":"2004-10-08T00:00:00Z","timestamp":1097193600000},"content-version":"vor","delay-in-days":3964,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of Neurology"],"published-print":{"date-parts":[[1993,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Here we describe a human T\u2010cell lymphotopic virus type I (HTLV\u2010I) seronegative patient from South India with a chronic, progressive spastic paraparesis from which HTLV\u2010I has been isolated from peripheral blood lymphocytes. HTLV\u2010I pol and tax viral sequences were detected in DNA from fresh peripheal blood lymphocytes (PBL) by polymerase chain reaction (PCR) and liquid hybridization techniques. Southern blot analysis of the PCR products demonstrated a low copy number of HTLV\u2010I at the level of one viral copy per 10,000 fresh PBL. A long\u2010term CD4<jats:sup>+<\/jats:sup> T\u2010cell line was established from PBL of this patient using recombinant interleukin\u20102, OKT3, and feeder cells. DNA from these cultured lines was amplified and portions of the HTLV\u2010I long terminal repeat (U3), pol, env, and tax regions were sequenced (a total of 1,115 bp). The sequence data showed that the HTLV\u2010I associated with this patient was 98.8% homologous to prototype HTLV\u2010I. Southern blot analysis also confirmed the presence of full\u2010length HTLV\u2010I. These results indicate that HTLV\u2010I can be demonstrated in an HTLV\u2010I seronegative patient from south India with a chronic progressive neurological disorder.<\/jats:p>","DOI":"10.1002\/ana.410340618","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T15:57:21Z","timestamp":1104595041000},"page":"867-870","source":"Crossref","is-referenced-by-count":12,"title":["Demonstration of human T\u2010cell lymphotrophic virus type I (HTLV\u2010I) from an HTLV\u2010I seronegative South Indian patient with chronic, progessive spastic paraparesis"],"prefix":"10.1002","volume":"34","author":[{"given":"Masataka","family":"Nishimura","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth","family":"Mingioli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dale E.","family":"McFarlin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven","family":"Jacobson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,10,8]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"245","volume-title":"Human retrovirology: HTLV","author":"Dalgleish AG","year":"1990"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410260404"},{"key":"e_1_2_1_4_2","volume-title":"Human retrovirology: HTLV","author":"McFarlin DE","year":"1990"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/331540a0"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.14.4544"},{"key":"e_1_2_1_7_2","doi-asserted-by":"crossref","first-page":"164","DOI":"10.2220\/biomedres.1.164","article-title":"Detection of immunoglobulin heavy chain genes accompanies the class switch rearrangement","volume":"1","author":"Yaoita Y","year":"1980","journal-title":"Biomed Res"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-372180-8.50043-3"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/312159a0"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.24.8818"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.80.12.3618"},{"key":"e_1_2_1_12_2","doi-asserted-by":"crossref","first-page":"1278","DOI":"10.1128\/jvi.64.3.1278-1282.1990","article-title":"Sequence variants of human T\u2010cell lymphotropic virus type I from patients with tropical spastic paraparesis and adult T\u2010cell leukemia do not distinguish neurological from leukemic isolates","volume":"64","author":"Daenke S","year":"1990","journal-title":"J Virol"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0404.1990.tb00968.x"},{"key":"e_1_2_1_14_2","doi-asserted-by":"crossref","unstructured":"BanghamCRM DaenkeS PhillipsRE et al . 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