{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T20:27:51Z","timestamp":1777580871190,"version":"3.51.4"},"reference-count":41,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2004,10,8]],"date-time":"2004-10-08T00:00:00Z","timestamp":1097193600000},"content-version":"vor","delay-in-days":3902,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of Neurology"],"published-print":{"date-parts":[[1994,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Sporadic amyotrophic lateral sclerosis is an idiopathic human degenerative disease of spinal cord and brain motor neurons. Prior studies demonstrated that most patients with amyotrophic lateral sclerosis posses immunoglobulins that bind to purified L\u2010type voltage\u2010gated calcium channels, that titers of anti\u2013voltage\u2010gated calcium channel antibodies correlate with disease progression rates, and that amyotrophic lateral sclerosis patient\u2010derived antibodies (ALS IgG) produce electrophysiological changes in the function of voltage\u2010gated calcium channels. Using Western transfer immunoblots and enzyme\u2010linked immunosorbent assays, the calcium ionophore\u2013forming \u03b1<jats:sub>1<\/jats:sub> subunig of the voltage\u2010gated calcium channel is now identified as the major voltage\u2010gated calcium channel antigen to which ALS IgG binds. Additionally, the binding of an L\u2010type voltage\u2010gated calcium channel \u03b1<jats:sub>1<\/jats:sub> subunit\u2013directed monoclonal antibody, which itself mimics the effects of ALS IgG on skeletal muscle voltage\u2010gated calcium channel currents, is selectively prevented by preaddition of ALS IgG. Voltage\u2010gated calcium channel\u2013binding IgG from patients with Lambert\u2010Eaton myasthenic syndrome appears to be differentiated from ALS IgG by the reactivity of the former to both \u03b1<jats:sub>1<\/jats:sub> and \u03b2 subunits of the calcium channel. These assays provide further evidence linking amyotrophic lateral sclerosis to an autoimmune process, and suggest one means to differentiate immunoglobulins from patients with amyotrophic lateral sclerosis from those of patients with another autoimmune disease expressing calcium channel antibodies.<\/jats:p>","DOI":"10.1002\/ana.410350207","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T15:59:54Z","timestamp":1104595194000},"page":"164-171","source":"Crossref","is-referenced-by-count":79,"title":["Amyotrophic lateral sclerosis patient antibodies label Ca<sup>2+<\/sup> channel \u03b1<sub>1<\/sub> subunit"],"prefix":"10.1002","volume":"35","author":[{"given":"Fumiharu","family":"Kimura","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. 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