{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T08:44:54Z","timestamp":1790930694909,"version":"4.1.0"},"reference-count":17,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2004,10,8]],"date-time":"2004-10-08T00:00:00Z","timestamp":1097193600000},"content-version":"vor","delay-in-days":3295,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of Neurology"],"published-print":{"date-parts":[[1995,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Cerebrospinal fluid from 70 patients with Alzheimer's disease (AD) and 96 patients with non\u2010AD neurological diseases as well as 19 normal control subjects was surveyed by sandwich enzyme\u2010linked immunosorbent assay to quantitate levels of the microtubule\u2010associated protein tau in cerebrospinal fluid. The tau level was significantly increased in AD patients as compared with that in patients with non\u2010AD neurological diseases and control subjects. Increased tau levels were found irrespective of age at onset, apolipoprotein E genotype, and clinical stage. Western blots of AD cerebrospinal fluid proteins revealed two to three tau\u2010immunoreactive bands with an apparent molecular mass between 50 and 65 kd consistent with phosphorylated cerebrospinal fluid tau. Taken together, our results suggest that cerebrospinal fluid tau might reflect the progressive accumulation of altered tau due to the progressive death of neurons in the AD brain, and that the enzyme\u2010linked immunosorbent assay of cerebrospinal fluid tau may prove to be a reliable and early diagnostic test for AD.<\/jats:p>","DOI":"10.1002\/ana.410380414","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T11:27:23Z","timestamp":1104578843000},"page":"649-652","source":"Crossref","is-referenced-by-count":227,"title":["Tau in cerebrospinal fluid: A potential diagnostic marker in Alzheimer's disease"],"prefix":"10.1002","volume":"38","author":[{"given":"Hiroyuki","family":"Arai","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masanori","family":"Terajima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masakazu","family":"Miura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susumu","family":"Higuchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taro","family":"Muramatsu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nobuo","family":"Machida","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hisatomo","family":"Seiki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sadao","family":"Takase","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher M.","family":"Clark","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Virginia M.\u2010Y.","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John Q.","family":"Trojanowski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hidetada","family":"Sasaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,10,8]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"crossref","first-page":"939","DOI":"10.1212\/WNL.34.7.939","article-title":"Clinical diagnosis of Alzheimer's disease: report of the NINCDS\u2010ADRDA Work Group under auspices of Department of Health and Human Services Task Force on Alzheimer's disease","volume":"34","author":"MeKhann G","year":"1984","journal-title":"Neurology"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1001\/archneur.1985.04060100083029"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410360616"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.7.2551"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00310917"},{"key":"e_1_2_1_7_2","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1046\/j.1471-4159.1995.64010443.x","article-title":"Elevated levels of the endosomal\u2010lysosomal proteinase cathepsin D in cerebrospinal fluid in Alzheimer's disease","volume":"64","author":"Schwargel AL","year":"1995","journal-title":"J Neurochem"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.7973660"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1899488"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0166-2236(93)90078-Z"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1176\/ajp.139.2.165"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1993.tb09823.x"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(94)90264-X"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.3083509"},{"key":"e_1_2_1_15_2","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1016\/S0021-9258(18)48531-6","article-title":"Hydrofluoric acid\u2010treated tau\/PHF proteins display the same biochemical properties as normal tau","volume":"267","author":"Greenberg SG","year":"1992","journal-title":"J Biol Chem"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(91)92823-K"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410370325"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(94)11110-5"}],"container-title":["Annals of Neurology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fana.410380414","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ana.410380414","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T14:06:03Z","timestamp":1698329163000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ana.410380414"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,10]]},"references-count":17,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1995,10]]}},"alternative-id":["10.1002\/ana.410380414"],"URL":"https:\/\/doi.org\/10.1002\/ana.410380414","archive":["Portico"],"relation":{},"ISSN":["0364-5134","1531-8249"],"issn-type":[{"value":"0364-5134","type":"print"},{"value":"1531-8249","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,10]]}}}