{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T07:13:15Z","timestamp":1789369995797,"version":"build-2803163510"},"reference-count":64,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2004,10,8]],"date-time":"2004-10-08T00:00:00Z","timestamp":1097193600000},"content-version":"vor","delay-in-days":2898,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of Neurology"],"published-print":{"date-parts":[[1996,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Barium currents through voltage\u2010gated calcium (Ca<jats:sup>2+<\/jats:sup>) channels were studied in the small\u2010cell lung carcinoma cell line NCI\u2010H345 using patch clamp techniques. Pharmacological dissection of whole\u2010cell barium currents revealed that 23% of the current was sensitive to nitrendipine, 35% to \u03c9\u2010conotoxin GVIA, and between 10 and 39% to \u03c9\u2010Aga\u2010IVA. This implies that these cells express L\u2010, N\u2010, and P\u2010type calcium channels. Only large cells expressed current that was sensitive to \u03c9\u2010Aga\u2010IVA. The size dependency of this P\u2010type channel expression may reflect the cell cycle stage. Cell\u2010attached recordings revealed three unitary conductances: 5 to 6 pS, 10 to 12 pS, and 20 to 23 pS. The largest conductance channel (20\u201023 pS) was sensitive to bay K 8644 and is presumed to represent L\u2010type calcium channels. The frequency of observing the medium conductance channel (10\u201012 pS) was reduced by exposure to \u03c9\u2010conotoxin GVIA and may represent N\u2010type channels. Incubation of cells with Lambert\u2010Eaton myasthenic syndrome IgG for 24 to 48 hours removed up to 71% of the whole\u2010cell current. Incubation with control human IgG (normal or myasthenia gravis) had no effect. Lambert\u2010Eaton maysthenic syndrome IgG did not selectively target one \u201cpresynaptic\u201d type of calcium channel, but rather appeared to target many of the calcium channel types that are expressed on small\u2010cell lung carcinoma cells.<\/jats:p>","DOI":"10.1002\/ana.410400510","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T14:52:41Z","timestamp":1104591161000},"page":"739-749","source":"Crossref","is-referenced-by-count":54,"title":["Lambert\u2010eaton myasthenic syndrome immunoglobulins react with multiple types of calcium channels in small\u2010cell lung carcinoma"],"prefix":"10.1002","volume":"40","author":[{"given":"Stephen D.","family":"Meriney","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan C.","family":"Hulsizer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alan 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