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The former results from IgG autoantibody\u2013mediated down\u2010regulation of P\/Q\u2010type voltage\u2010gated calcium channels at motor nerve terminals and consequent reduction in acetylcholine release; the basis for the autonomic symptoms is unknown. Using \u03c9\u2010conotoxins GVIA and MVIIC and \u03c9\u2010agatoxin IVA that block N\u2010, Q\u2010, and P\u2010type channels, we investigated ex vivo the calcium channels subserving transmitter release from postganglionic parasympathetic neurons in the bladder and from postganglionic sympathetic neurons in the vas deferens of mice injected with IgG from LEMS patients or from controls. Calcium influx through N\u2010, P\u2010, and Q\u2010type channels subserved transmitter release from parasympathetic and sympathetic neurons in control mice. In test mice, the component of transmitter release subserved by P\u2010type channels was abolished by four of four LEMS IgG preparations, that subserved by Q\u2010type channels was significantly reduced by three, and that subserved by N\u2010type channels by one. Thus, LEMS IgG impairs transmitter release from parasympathetic and sympathetic neurons through down\u2010regulation of one or more subtypes of voltage\u2010gated calcium channels. The results suggest that antibody\u2010mediated interference with specific ion channel function may also underlie autonomic dysfunction occurring in other autoimmune diseases.<\/jats:p>","DOI":"10.1002\/ana.410420204","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T15:04:12Z","timestamp":1104591852000},"page":"147-156","source":"Crossref","is-referenced-by-count":89,"title":["Effect of Lambert\u2010Eaton myasthenic syndrome antibodies on autonomic neurons in the mouse"],"prefix":"10.1002","volume":"42","author":[{"given":"Sally A.","family":"Waterman","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bethan","family":"Lang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"Newsom\u2010Davis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,10,8]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/111.3.577"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00194096"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/317737a0"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/mus.880050905"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.80.24.7636"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0166-2236(95)80030-6"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199506013322203"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410290313"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1136\/jnnp.58.1.85"},{"key":"e_1_2_1_11_2","doi-asserted-by":"crossref","unstructured":"MotomuraM LangB JohnstonI et al. 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