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Additionally, the strategies for mimicking natural cargo exchange mostly rely on biological techniques, which involve integrating native channel proteins or adopting intact natural cell membranes. Herein, we propose a rational chemical design strategy to construct artificial mini cells (MCs) based on a lipid\u2013polymer integrated architecture. Our MCs show three key merits, including robust structural stability, controllable metabolic reactions, and convenient surface functionalization for diverse biomedical applications. MCs adopt a lipid\u2013polymer integrated architecture, where a cross\u2010linked zwitterionic polymer in situ grown on the phospholipid membrane acts as the conceptually synthesized cytoskeleton mimic, ensuring structural stability and easy functionalization. Azobenzene\u2010gated lipids are incorporated to act as light\u2010responsive channel protein mimics, enabling spatiotemporal regulation of membrane permeability and metabolic reactions. As a proof of concept, therapeutic artificial MCs with tumor tropism, surface\u2010immobilized aPDL1 proteins, and internally encapsulated glucose oxidase\u2013catalase (GOx\u2013Cat) multi\u2010enzyme system were rationally designed, named aPDL1\u2010Tt\u2010MC(GOx + Cat). aPDL1\u2010Tt\u2010MC(GOx + Cat)s possess zwitterionic ionizable pyridine carboxybetaine moieties for tumor tropism, the GOx\u2013Cat system for light\u2010controlled glucose\u2010to\u2010O\n                    <jats:sub>2<\/jats:sub>\n                    conversion to drive deep tumor infiltration, and surface\u2010immobilized aPDL1 to block PD\u20101\/PD\u2010L1 recognition for enhanced antitumor efficacy.\n                  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