{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T18:40:21Z","timestamp":1792262421815,"version":"4.4.0"},"reference-count":45,"publisher":"Wiley","issue":"43","license":[{"start":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T00:00:00Z","timestamp":1788912000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T00:00:00Z","timestamp":1788912000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Angew Chem Int Ed"],"published-print":{"date-parts":[[2026,10,19]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Chemical exchange saturation transfer (CEST) MRI enables sensitive detection of low\u2010concentration metabolites in vivo, but its biological interpretation is limited by the composite nature of the signal, which reflects contributions from multiple cell types. In the brain, these include intracellular metabolites in neurons and glial cells, as well as semisolid macromolecular pools. Here, we introduce MangaCEST, a strategy that uses manganese (Mn\n                    <jats:sup>2<\/jats:sup>\n                    <jats:sup>+<\/jats:sup>\n                    )\u2010induced intracellular relaxation changes to modulate the contributions of different cell populations to the CEST spectrum. Mn\n                    <jats:sup>2<\/jats:sup>\n                    <jats:sup>+<\/jats:sup>\n                    enters the cytoplasm via calcium\u2010permeable channels and accumulates in excitable cells in an activity\u2010dependent manner, shortening water T\n                    <jats:sub>1<\/jats:sub>\n                    and selectively reducing CEST contrast. Comparison of CEST Z\u2010spectra with or without Mn\n                    <jats:sup>2<\/jats:sup>\n                    <jats:sup>+<\/jats:sup>\n                    enables MangaCEST to isolate Mn\u2010sensitive signal components and differentially analyze intracellular contributions. To provide a cell\u2010type\u2010weighted Mn\n                    <jats:sup>2<\/jats:sup>\n                    <jats:sup>+<\/jats:sup>\n                    effect in a well\u2010defined biological framework, we focused on the cerebellum, where preferential neuronal accumulation of Mn\n                    <jats:sup>2<\/jats:sup>\n                    <jats:sup>+<\/jats:sup>\n                    is well documented. In the developing chick embryo in vivo, Mn\n                    <jats:sup>2<\/jats:sup>\n                    <jats:sup>+<\/jats:sup>\n                    caused significant T\n                    <jats:sub>1<\/jats:sub>\n                    shortening (\u223c28%) and frequency\u2010dependent CEST spectrum changes. Differential spectra showed attenuation at 3\u20134\u00a0ppm, consistent with contributions from neuronal\u2010associated metabolite pools. These findings suggest that MangaCEST may represent a promising strategy for disentangling cellular contributions to molecular MRI signals in the brain.\n                  <\/jats:p>","DOI":"10.1002\/anie.1291856","type":"journal-article","created":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T14:16:56Z","timestamp":1788963416000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Toward Cell\u2010Specific Molecular MRI: Modulating Chemical Exchange Saturation Transfer Contrast With Manganese"],"prefix":"10.1002","volume":"65","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4891-4138","authenticated-orcid":false,"given":"Eleonora","family":"Cavallari","sequence":"first","affiliation":[{"name":"Department of Molecular Biotechnology and Health Sciences University of Torino  Torino Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2138-0658","authenticated-orcid":false,"given":"Valeria","family":"Bitonto","sequence":"additional","affiliation":[{"name":"Department of Molecular Biotechnology and Health Sciences University of Torino  Torino Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-9385-5123","authenticated-orcid":false,"given":"Morgana","family":"Giachello","sequence":"additional","affiliation":[{"name":"Department of Molecular Biotechnology and Health Sciences University of Torino  Torino Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7423-3419","authenticated-orcid":false,"given":"Angelo","family":"Bifone","sequence":"additional","affiliation":[{"name":"Department of Molecular Biotechnology and Health Sciences University of Torino  Torino 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